Amitriptyline down-regulates coenzyme Q10 biosynthesis in lung cancer cells

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dc.contributor.authorOrtiza, Tamara
dc.contributor.authorVillanueva-Pazc, Marina
dc.contributor.authorDíaz-Parradoa, Eduardo
dc.contributor.authorIllanesa, Matilde
dc.contributor.authorFernández-Rodríguez, Ana
dc.contributor.authorSánchez-Alcázarc, José A
dc.contributor.authorde Miguel, Manuel
dc.date.accessioned2022-01-17T21:41:11Z
dc.date.available2022-01-17T21:41:11Z
dc.date.issued2017
dc.description.abstractAmitriptyline, a tricyclic antidepressant, has been proposed as an antitumoral drug in oxidative therapy. Its proapoptotic effects, mediated by high reactive oxygen species generation, have been already described. In this study we analysed the effect of amitriptyline on the biosynthesis of coenzyme Q10 (CoQ), an essential component for electron transport and a potent membrane antioxidant involved in redox signaling. We treated H460 cells, a non-small-cell lung cancer cell line, with amitriptyline and we analysed CoQ levels by HPLC and CoQ biosynthesis rate, as well as the enzymes involved in CoQ biosynthesis by real-time PCR and Western blot. Amitriptyline treatment induced a dose-dependent decrease in CoQ levels in tumor cells. CoQ decreased levels were associated with down-regulation of the expression of COQ4 gene, as well as decreased Coq4 and Coq6 protein levels. Our findings suggest that the effect of amitriptyline on CoQ biosynthesis highlights the potential of this drug for antitumoral oxidative therapyes
dc.identifier.citationOrtiz T, Villanueva-Paz M, Díaz-Parrado E, Illanes M, Fernández-Rodríguez A, Sánchez-Alcázar JA, de Miguel M. Amitriptyline down-regulates coenzyme Q10 biosynthesis in lung cancer cells. Eur J Pharmacol. 2017 Feb 15;797:75-82.es
dc.identifier.urihttp://dx.doi.org/10.1016/j.ejphar.2017.01.017. Epub 2017 Jan 15.es
dc.identifier.urihttp://hdl.handle.net/11447/5472
dc.language.isoen_USes
dc.subjectAmitriptylinees
dc.subjectCoenzyme Q10es
dc.subjectOxidative stresses
dc.subjectAntioxidantses
dc.subjectOxidative therapyes
dc.titleAmitriptyline down-regulates coenzyme Q10 biosynthesis in lung cancer cellses
dc.typeArticlees

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