Palmitic and Stearic Acids Inhibit Chaperone-Mediated Autophagy (CMA) in POMC-like Neurons In Vitro

Simple item page
dc.contributor.authorEspinosa, Rodrigo
dc.contributor.authorGutiérrez, Karla
dc.contributor.authorRios, Javiera
dc.contributor.authorOrmeño, Fernando
dc.contributor.authorYantén, Liliana
dc.contributor.authorCésar A. Ramírez-Sarmiento
dc.contributor.authorGalaz, Pablo
dc.contributor.authorRamírez, César
dc.contributor.authorParra, Valentina
dc.contributor.authorAlbornoz, Amelina
dc.contributor.authorAlfaro, Iván
dc.contributor.authorBurgos, Patricia
dc.contributor.authorMorselli, Eugenia
dc.contributor.authorCriollo, Alfredo
dc.contributor.authorBudini, Mauricio
dc.date.accessioned2022-03-08T20:52:32Z
dc.date.available2022-03-08T20:52:32Z
dc.date.issued2022
dc.description.abstractThe intake of food with high levels of saturated fatty acids (SatFAs) is associated with the development of obesity and insulin resistance. SatFAs, such as palmitic (PA) and stearic (SA) acids, have been shown to accumulate in the hypothalamus, causing several pathological conse-quences. Autophagy is a lysosomal-degrading pathway that can be divided into macroautophagy, microautophagy, and chaperone-mediated autophagy (CMA). Previous studies showed that PA im-pairs macroautophagy function and insulin response in hypothalamic proopiomelanocortin (POMC) neurons. Here, we show in vitro that the exposure of POMC neurons to PA or SA also inhibits CMA, possibly by decreasing the total and lysosomal LAMP2A protein levels. Proteomics of lysosomes from PA- and SA-treated cells showed that the inhibition of CMA could impact vesicle formation and trafficking, mitochondrial components, and insulin response, among others. Finally, we show that CMA activity is important for regulating the insulin response in POMC hypothalamic neurons. These in vitro results demonstrate that CMA is inhibited by PA and SA in POMC-like neurons, giving an overview of the CMA-dependent cellular pathways that could be affected by such inhibition and opening a door for in vivo studies of CMA in the context of the hypothalamus and obesity.es
dc.description.versionVersión publicadaes
dc.identifier.citationEspinosa, R.; Gutiérrez, K.; Rios, J.; Ormeño, F.; Yantén, L.; Galaz-Davison, P.; Ramírez-Sarmiento, C.A.; Parra, V.; Albornoz, A.; Alfaro, I.E.; et al. Palmitic and Stearic Acids Inhibit Chaperone-Mediated Autophagy (CMA) in POMC-like Neurons In Vitro. Cells 2022, 11, 920. https:// doi.org/10.3390/cells11060920es
dc.identifier.urihttps://doi.org/10.3390/cells11060920es
dc.identifier.urihttp://hdl.handle.net/11447/5680
dc.language.isoenes
dc.subjectchaperone-mediated autophagy (CMA)es
dc.subjectinsulines
dc.subjectpalmitices
dc.subjectstearices
dc.subjectlysosomees
dc.subjectobesityes
dc.subjectproteomicses
dc.subjectSILACes
dc.titlePalmitic and Stearic Acids Inhibit Chaperone-Mediated Autophagy (CMA) in POMC-like Neurons In Vitroes
dc.typeArticlees
dcterms.sourceCellses

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Palmitic and Stearic.pdf
Size:
5.08 MB
Format:
Adobe Portable Document Format
Description:
Texto completo
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services