Tissuepatch is biocompatible and seals iatrogenic membrane defects in a rabbit model
Date
2018
Type:
Article
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Abstract
Objective: To evaluate novel sealing techniques for their biocompatibility and sealing capacity of iatrogenic fetal membrane defects in a pregnant rabbit model.
Method: At day 23 of gestation (term = d31), a standardized fetoscopy was performed through a 14G cannula. The resulting fetal membrane defect was closed with condensed collagen, collagen with fibrinogen, Tissuepatch, Duraseal, or a conventional collagen plug (Lyostypt) as reference. At d30, the fetuses were harvested and full thickness fetal membrane samples were analyzed. The study consisted of 2 consecutive parts: (1) biocompatibility testing by fetal survival, apoptosis, and infiltration of polymorphonuclear cells in the membranes and (2) the efficacy to seal fetal membrane defects.
Results: Three sealants (collagen with fibrinogen, Duraseal, or Lyostypt) were associated with a higher fetal mortality compared to control unmanipulated littermates and hence were excluded from further analysis. Tissuepatch was biocompatible, and amniotic fluid levels were comparable to those of control untouched littermates. Compared to the condensed collagen, Tissuepatch was also easier in surgical handling and induced limited cell proliferation.
Conclusion: Tissuepatch had the best biocompatibility and efficacy in sealing an iatrogenic fetal membrane defect in the pregnant rabbit compared to other readily available sealants.
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Citation
Engels AC, Joyeux L, Van der Merwe J, Jimenez J, Pranpanus S, Barrett DW, Connon C, Chowdhury TT, David AL, Deprest J. Tissuepatch is biocompatible and seals iatrogenic membrane defects in a rabbit model. Prenat Diagn. 2018 Jan;38(2):99-105. doi: 10.1002/pd.5191
Keywords
Extraembryonic Membranes / abnormalities, Extraembryonic Membranes / chemistry, Extraembryonic Membranes / surgery, Fetal Membranes, Premature Rupture / prevention & control, Fetal Mortality, In Situ Nick-End Labeling, Iatrogenic Disease, Disease Models, Animal, Collagen