Genomic data mining of the marine actinobacteria Streptomyces sp H-KF8 unveils insights into multi- stress related genes and metabolic pathways involved in antimicrobial synthesis

Simple item page
dc.contributor.authorUndabarrena, Agustina
dc.contributor.authorUgalde, Juan
dc.contributor.authorSeeger, Michael
dc.contributor.authorCámara, Beatriz
dc.date.accessioned2018-01-19T15:09:04Z
dc.date.available2018-01-19T15:09:04Z
dc.date.issued2017
dc.description.abstractStreptomyces sp. H-KF8 is an actinobacterial strain isolated from marine sediments of a Chilean Patagonian fjord. Morphological characterization together with antibacterial activity was assessed in various culture media, revealing a carbon-source dependent activity mainly against Gram-positive bacteria (S. aureus and L. monocytogenes). Genome mining of this antibacterial-producing bacterium revealed the presence of 26 biosynthetic gene clusters (BGCs) for secondary metabolites, where among them, 81% have low similarities with known BGCs. In addition, a genomic search in Streptomyces sp. H-KF8 unveiled the presence of a wide variety of genetic determinants related to heavy metal resistance (49 genes), oxidative stress (69 genes) and antibiotic resistance (97 genes). This study revealed that the marine-derived Streptomyces sp. H-KF8 bacterium has the capability to tolerate a diverse set of heavy metals such as copper, cobalt, mercury, chromate and nickel; as well as the highly toxic tellurite, a feature first time described for Streptomyces. In addition, Streptomyces sp. H-KF8 possesses a major resistance towards oxidative stress, in comparison to the soil reference strain Streptomyces violaceoruber A3(2). Moreover, Streptomyces sp. H-KF8 showed resistance to 88% of the antibiotics tested, indicating overall, a strong response to several abiotic stressors. The combination of these biological traits confirms the metabolic versatility of Streptomyces sp. H-KF8, a genetically well-prepared microorganism with the ability to confront the dynamics of the fjord-unique marine environment.
dc.format.extent35
dc.identifier.citationPeerJ. 2017 Feb 14;5:e2912
dc.identifier.urihttp://hdl.handle.net/11447/1925
dc.identifier.urihttp://dx.doi.org/10.1590/10.7717/peerj.2912
dc.language.isoen_US
dc.publisherPeerJ Inc.
dc.subjectMarine actinomycete
dc.subjectGenome mining
dc.subjectStreptomyces
dc.subjectBiosynthethic gene clusters
dc.subjectAntimicrobial activity
dc.subjectHeavy metal tolerance
dc.subjectAbiotic stressors
dc.subjectChilean Patagonian fjord
dc.titleGenomic data mining of the marine actinobacteria Streptomyces sp H-KF8 unveils insights into multi- stress related genes and metabolic pathways involved in antimicrobial synthesis
dc.typeArtículo

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Genomic data mining of the marine actinobacteria Streptomyces sp H-KF8 unveils insights into multi- stress related genes and metabolic pathways involved in antimicrobial synthesis___completo.pdf
Size:
15.39 MB
Format:
Adobe Portable Document Format
Description:
Texto completo
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services