Genomic data mining of the marine actinobacteria Streptomyces sp H-KF8 unveils insights into multi- stress related genes and metabolic pathways involved in antimicrobial synthesis
Date
2017
Type:
Artículo
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35
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Volume Title
Publisher
PeerJ Inc.
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Abstract
Streptomyces sp. H-KF8 is an actinobacterial strain isolated from marine sediments of
a Chilean Patagonian fjord. Morphological characterization together with antibacterial
activity was assessed in various culture media, revealing a carbon-source dependent activity
mainly against Gram-positive bacteria (S. aureus and L. monocytogenes). Genome
mining of this antibacterial-producing bacterium revealed the presence of 26 biosynthetic
gene clusters (BGCs) for secondary metabolites, where among them, 81% have
low similarities with known BGCs. In addition, a genomic search in Streptomyces sp.
H-KF8 unveiled the presence of a wide variety of genetic determinants related to
heavy metal resistance (49 genes), oxidative stress (69 genes) and antibiotic resistance
(97 genes). This study revealed that the marine-derived Streptomyces sp. H-KF8
bacterium has the capability to tolerate a diverse set of heavy metals such as copper,
cobalt, mercury, chromate and nickel; as well as the highly toxic tellurite, a feature
first time described for Streptomyces. In addition, Streptomyces sp. H-KF8 possesses
a major resistance towards oxidative stress, in comparison to the soil reference strain
Streptomyces violaceoruber A3(2). Moreover, Streptomyces sp. H-KF8 showed resistance
to 88% of the antibiotics tested, indicating overall, a strong response to several abiotic
stressors. The combination of these biological traits confirms the metabolic versatility
of Streptomyces sp. H-KF8, a genetically well-prepared microorganism with the ability
to confront the dynamics of the fjord-unique marine environment.
Description
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Citation
PeerJ. 2017 Feb 14;5:e2912
Keywords
Marine actinomycete, Genome mining, Streptomyces, Biosynthethic gene clusters, Antimicrobial activity, Heavy metal tolerance, Abiotic stressors, Chilean Patagonian fjord