Renal peritumoral adipose tissue undergoes a browning process and stimulates the expression of epithelial-mesenchymal transition markers in human renal cells

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dc.contributor.authorFerrando, Matías
dc.contributor.authorBruna, Flavia
dc.contributor.authorRomeo, Leonardo
dc.contributor.authorContador, David
dc.contributor.authorMoya, Daiana
dc.contributor.authorSantiano, Flavia
dc.contributor.authorZyla, Leila
dc.contributor.authorGomez, Silvina
dc.contributor.authorFontana, Constanza
dc.contributor.authorCalvo, Juan
dc.contributor.authorCarón, Rubén
dc.contributor.authorToneatto, Judith
dc.contributor.authorPistone, Virginia
dc.date.accessioned2023-03-07T11:46:28Z
dc.date.available2023-03-07T11:46:28Z
dc.date.issued2022
dc.description.abstractTumor cells can interact with neighboring adipose cells and adipocyte dedifferentiation appears to be an important aspect of tumorigenesis. We evaluated the size of adipocytes in human adipose explants from normal (hRAN) and kidney cancer (hRAT); changes in the expression of WAT and BAT/beige markers in hRAN and hRAT; the expression of epithelial-mesenchymal transition (EMT) cell markers in human kidney tumor (786-O, ACHN and Caki-1); and non-tumor (HK-2) epithelial cell lines incubated with the conditioned media (CMs) of hRAN and hRAT. We observed that hRAT adipocytes showed a significantly minor size compared to hRAN adipocytes. Also, we observed that both Prdm16 and Tbx1 mRNA and the expression of UCP1, TBX1, PPARγ, PCG1α, c/EBPα LAP and c/EBPα LIP was significantly higher in hRAT than hRAN. Finally, we found an increase in vimentin and N-cadherin expression in HK-2 cells incubated for 24 h with hRAT-CMs compared to hRAN- and control-CMs. Furthermore, desmin and N-cadherin expression also increased significantly in 786-O when these cells were incubated with hRAT-CMs compared to the value observed with hRAN- and control-CMs. We observed a significant decrease in E-cadherin expression in the ACHN cell line incubated with hRAT-CMs versus hRAN- and control-CMs. However, we did not observe changes in E-cadherin expression in HK-2, 786-O or Caki-1. The results obtained, together with the results previously published by our group, allow us to conclude that perirenal white adipose tissue browning contributes to tumor development in kidney cancer. In addition, hRAT-CMs increases the expression of mesenchymal markers in renal epithelial cells, which could indicate a regulation of EMT due to this adipose tissue.
dc.description.versionVersión publicada
dc.identifier.citationFerrando M, Bruna FA, Romeo LR, Contador D, Moya-Morales DL, Santiano F, Zyla L, Gomez S, Lopez-Fontana CM, Calvo JC, Carón RW, Toneatto J, Pistone-Creydt V. Renal peritumoral adipose tissue undergoes a browning process and stimulates the expression of epithelial-mesenchymal transition markers in human renal cells. Sci Rep. 2022 May 23;12(1):8687. doi: 10.1038/s41598-022-12746-9
dc.identifier.doihttps://doi.org/10.1038/s41598-022-12746-9
dc.identifier.urihttps://repositorio.udd.cl/handle/11447/7073
dc.language.isoen
dc.subjectAdipose Tissue / metabolism
dc.subjectAdipose Tissue, Brown / metabolism
dc.subjectAdipose Tissue, White / metabolism
dc.subjectCadherins / metabolism
dc.subjectEpithelial-Mesenchymal Transition
dc.subjectKidney Neoplasms / genetics
dc.subjectKidney Neoplasms / metabolism
dc.titleRenal peritumoral adipose tissue undergoes a browning process and stimulates the expression of epithelial-mesenchymal transition markers in human renal cells
dc.typeArticle
dcterms.sourceScientific reports

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