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Mutación del gen BRAF en pacientes con cánceres de colon y recto con KRAS no mutado

Show simple item record Roa, Iván Game, Anakaren Bizama, Carolina Schalper, Kurt 2017-03-02T18:27:25Z 2017-03-02T18:27:25Z 2014
dc.identifier.citation Revista Médica de Chile 2014, vol.142,p.55-60
dc.description.abstract Background: In colorectal cancer, BRAF and KRAS mutation are mutually exclusive, but both are independent prognostic factors for the disease. Aim: To determine the frequency of BRAF V600E mutation in colorectal cancer. Material and Methods: A KRAS mutation study was carried out in 100 tissue samples of primary and metastatic adenocarcinomas of colon and rectum from patients aged 61.1 ± 62 years (56 women). Negative KRAS mutation cases underwent study of BRAF V600E mutation by restriction fragment length polymorphism (RFLP) and direct sequencing. Results: Primary tumors were located in the colon and rectum in 88 and six cases respectively. Five were liver metastases and in one case, the sample location was undetermined. Forty two samples were KRAS positive (mutated). In 12 of the 58 KRAS negative (wild type) samples, the V600E mutation in codon 15 of the BRAF gene was demonstrated. No differences in the frequency and distribution of mutations, stratified by gender, age, primary tumor versus metastasis, or tumor location were observed. Conclusions: Twelve percent of KRAS negative colorectal cancer samples showed BRAF gene mutation. Considering that 42% of samples have a KRAS mutation, 54% of patients should not respond to therapies with monoclonal antibodies directed against epidermic growth factor (EGFR) pathway.
dc.format.extent 6
dc.language.iso spa
dc.publisher Sociedad Médica de Santiago
dc.subject Proto-Oncogene Proteins p21 (ras)
dc.subject Proto-Oncogene Proteins B-raf
dc.subject Mutation
dc.subject Colonic neoplasms
dc.title Mutación del gen BRAF en pacientes con cánceres de colon y recto con KRAS no mutado
dc.title.alternative BRAF gene mutation in wild-type KRAS patients with colorectal cancers
dc.type Artículo

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