Mutación del gen BRAF en pacientes con cánceres de colon y recto con KRAS no mutado

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dc.contributor.authorRoa, Iván
dc.contributor.authorGame, Anakaren
dc.contributor.authorBizama, Carolina
dc.contributor.authorSchalper, Kurt
dc.date.accessioned2017-03-02T18:27:25Z
dc.date.available2017-03-02T18:27:25Z
dc.date.issued2014
dc.description.abstractBackground: In colorectal cancer, BRAF and KRAS mutation are mutually exclusive, but both are independent prognostic factors for the disease. Aim: To determine the frequency of BRAF V600E mutation in colorectal cancer. Material and Methods: A KRAS mutation study was carried out in 100 tissue samples of primary and metastatic adenocarcinomas of colon and rectum from patients aged 61.1 ± 62 years (56 women). Negative KRAS mutation cases underwent study of BRAF V600E mutation by restriction fragment length polymorphism (RFLP) and direct sequencing. Results: Primary tumors were located in the colon and rectum in 88 and six cases respectively. Five were liver metastases and in one case, the sample location was undetermined. Forty two samples were KRAS positive (mutated). In 12 of the 58 KRAS negative (wild type) samples, the V600E mutation in codon 15 of the BRAF gene was demonstrated. No differences in the frequency and distribution of mutations, stratified by gender, age, primary tumor versus metastasis, or tumor location were observed. Conclusions: Twelve percent of KRAS negative colorectal cancer samples showed BRAF gene mutation. Considering that 42% of samples have a KRAS mutation, 54% of patients should not respond to therapies with monoclonal antibodies directed against epidermic growth factor (EGFR) pathway.
dc.format.extent6
dc.identifier.citationRevista Médica de Chile 2014, vol.142,p.55-60
dc.identifier.urihttp://hdl.handle.net/11447/987
dc.identifier.urihttp://dx.doi.org/10.4067/S0034-98872014000100009
dc.language.isospa
dc.publisherSociedad Médica de Santiago
dc.subjectProto-Oncogene Proteins p21 (ras)
dc.subjectProto-Oncogene Proteins B-raf
dc.subjectMutation
dc.subjectColonic neoplasms
dc.titleMutación del gen BRAF en pacientes con cánceres de colon y recto con KRAS no mutado
dc.title.alternativeBRAF gene mutation in wild-type KRAS patients with colorectal cancers
dc.typeArtículo

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