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Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score

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dc.contributor.author Tekin, Nilgun
dc.contributor.author Omidvar, Nader
dc.contributor.author Morris, Tim Peter
dc.contributor.author Conget, Paulette
dc.contributor.author Bruna, Flavia
dc.contributor.author Timar, Botond
dc.contributor.author Gagyi, Eva
dc.contributor.author Basak, Ranjan
dc.contributor.author Naik, Omkar
dc.contributor.author Auewarakul, Chirayu
dc.contributor.author Sritana, Narongrit
dc.contributor.author Levy, Debora
dc.contributor.author Cerci, Juliano
dc.contributor.author Bydlowski, Sergio Paulo
dc.contributor.author Pereira, Juliana
dc.contributor.author Dimamay, Mark Pierre
dc.contributor.author Natividad, Filipinas
dc.contributor.author Chung, June-Key
dc.contributor.author Belder, Nevin
dc.contributor.author Kuzu, Isinsu
dc.contributor.author Paez, Diana
dc.contributor.author Dondi, Maurizio
dc.contributor.author Carr, Robert
dc.contributor.author Ozdag, Hilal
dc.contributor.author Padua, Rose Ann
dc.date.accessioned 2017-08-10T20:38:37Z
dc.date.available 2017-08-10T20:38:37Z
dc.date.issued 2016
dc.identifier.citation Oncotarget. 2016 Dec 13; 7(50): 83319–83329
dc.identifier.uri http://hdl.handle.net/11447/1563
dc.identifier.uri http://dx.doi.org/10.18632/oncotarget.13066
dc.description.abstract As a part of an international study on the molecular analysis of Diffuse Large B-cell Lymphoma (DLBCL), a robust protocol for gene expression analysis from RNA extraction to qRT-PCR using Formalin Fixed Paraffin Embedded tissues was developed. Here a study was conducted to define a strategy to validate the previously reported 6-gene (LMO2, BCL6, FN1, CCND2, SCYA3 and BCL2) model as predictor of prognosis in DLBCL. To avoid variation, all samples were tested in a single centre and single platform. This study comprised 8 countries (Brazil, Chile, Hungary, India, Philippines, S. Korea, Thailand and Turkey). Using the Kaplan-Meier and log rank test on patients (n=162) and two mortality risk groups (with those above and below the mean representing high and low risk groups) confirmed that the 6-gene predictor score correlates significantly with overall survival (OS, p<0.01) but not with event free survival (EFS, p=0.18). Adding the International Prognostic Index (IPI) shows that the 6-gene predictor score correlates significantly with high IPI scores for OS (p<0.05), whereas those with low IPI scores show a trend not reaching significance (p=0.08). This study defined an effective and economical qRT-PCR strategy and validated the 6-gene score as a predictor of OS in an international setting.
dc.format.extent 11
dc.language.iso en_US
dc.publisher Impact Journals
dc.subject diffuse large B-cell lymphoma (DLBCL)
dc.subject 6-gene predictor score
dc.subject formalin fixed paraffin embedded tissue (FFPE)
dc.title Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score
dc.type Artículo


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