Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score

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dc.contributor.authorTekin, Nilgun
dc.contributor.authorOmidvar, Nader
dc.contributor.authorMorris, Tim Peter
dc.contributor.authorConget, Paulette
dc.contributor.authorBruna, Flavia
dc.contributor.authorTimar, Botond
dc.contributor.authorGagyi, Eva
dc.contributor.authorBasak, Ranjan
dc.contributor.authorNaik, Omkar
dc.contributor.authorAuewarakul, Chirayu
dc.contributor.authorSritana, Narongrit
dc.contributor.authorLevy, Debora
dc.contributor.authorCerci, Juliano
dc.contributor.authorBydlowski, Sergio Paulo
dc.contributor.authorPereira, Juliana
dc.contributor.authorDimamay, Mark Pierre
dc.contributor.authorNatividad, Filipinas
dc.contributor.authorChung, June-Key
dc.contributor.authorBelder, Nevin
dc.contributor.authorKuzu, Isinsu
dc.contributor.authorPaez, Diana
dc.contributor.authorDondi, Maurizio
dc.contributor.authorCarr, Robert
dc.contributor.authorOzdag, Hilal
dc.contributor.authorPadua, Rose Ann
dc.date.accessioned2017-08-10T20:38:37Z
dc.date.available2017-08-10T20:38:37Z
dc.date.issued2016
dc.description.abstractAs a part of an international study on the molecular analysis of Diffuse Large B-cell Lymphoma (DLBCL), a robust protocol for gene expression analysis from RNA extraction to qRT-PCR using Formalin Fixed Paraffin Embedded tissues was developed. Here a study was conducted to define a strategy to validate the previously reported 6-gene (LMO2, BCL6, FN1, CCND2, SCYA3 and BCL2) model as predictor of prognosis in DLBCL. To avoid variation, all samples were tested in a single centre and single platform. This study comprised 8 countries (Brazil, Chile, Hungary, India, Philippines, S. Korea, Thailand and Turkey). Using the Kaplan-Meier and log rank test on patients (n=162) and two mortality risk groups (with those above and below the mean representing high and low risk groups) confirmed that the 6-gene predictor score correlates significantly with overall survival (OS, p<0.01) but not with event free survival (EFS, p=0.18). Adding the International Prognostic Index (IPI) shows that the 6-gene predictor score correlates significantly with high IPI scores for OS (p<0.05), whereas those with low IPI scores show a trend not reaching significance (p=0.08). This study defined an effective and economical qRT-PCR strategy and validated the 6-gene score as a predictor of OS in an international setting.
dc.format.extent11
dc.identifier.citationTekin N, Omidvar N, Morris TP, Conget P, Bruna F, Timar B, Gagyi E, Basak R, Naik O, Auewarakul C, Sritana N, Levy D, Cerci JJ, Bydlowski SP, Pereira J, Dimamay MP, Natividad F, Chung JK, Belder N, Kuzu I, Paez D, Dondi M, Carr R, Ozdag H, Padua RA. Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score. Oncotarget. 2016 Dec 13;7(50):83319-83329
dc.identifier.urihttp://hdl.handle.net/11447/1563
dc.identifier.urihttp://dx.doi.org/10.18632/oncotarget.13066
dc.language.isoen_US
dc.publisherImpact Journals
dc.subjectdiffuse large B-cell lymphoma (DLBCL)
dc.subject6-gene predictor score
dc.subjectformalin fixed paraffin embedded tissue (FFPE)
dc.titleProtocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score
dc.typeArtículo

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