Characterization of diabetic neuropathy progression in a mouse model of type 2 diabetes mellitus

Simple item page
dc.contributor.authorGregorio, Cristian De
dc.contributor.authorContador, David
dc.contributor.authorCampero, Mario
dc.contributor.authorEzquer, Marcelo
dc.contributor.authorEzquer, Fernando
dc.date.accessioned2019-07-18T21:02:35Z
dc.date.available2019-07-18T21:02:35Z
dc.date.issued2018
dc.description.abstractDiabetes mellitus (DM) is one of most common chronic diseases with an increasing incidence in most countries. Diabetic neuropathy (DN) is one of the earliest and main complications of diabetic patients, which is characterized by progressive, distal-to-proximal degeneration of peripheral nerves. The cellular and molecular mechanisms that trigger DN are highly complex, heterogeneous and not completely known. Animal models have constituted a valuable tool for understanding diabetes pathophysiology; however, the temporal course of DN progression in animal models of type 2 diabetes (T2DM) is not completely understood. In this work, we characterized the onset and progression of DN in BKS diabetic (db/db) mice, including the main functional and histological features observed in the human disease. We demonstrated that diabetic animals display progressive sensory loss and electrophysiological impairments in the early-to-mid phases of the disease. Furthermore, we detected an early decrease in intraepidermal nerve fiber (IENF) density in 18-week-old diabetic mice, which is highly associated with sensory loss and constitutes a reliable marker of DN. Other common histological parameters of DN – like Schwann cells apoptosis and infiltration of CD3+ cells in the sciatic nerve – were altered in mid-to-late phases of the disease. Our results support the general consensus that DN evolves from initial functional to late structural changes. This work aimed to characterize the progression of DN in a reliable animal model sharing the main human disease features, which is necessary to assess new therapies for this complex disease. Finally, we also aimed to identify an effective temporal window where these potential treatments could be successfully applied.
dc.format.extent11 p.
dc.identifier.citationBiology Open, 2018, n° 7, 11 p.
dc.identifier.urihttp://hdl.handle.net/11447/2533
dc.identifier.urihttp:dx.doi.org/10.1242/bio.036830
dc.language.isoen
dc.subjectDiabetic neuropathy
dc.subjectType 2 diabetes mellitus
dc.subjectAnimal model
dc.subjectPeripheral nerves
dc.subjectAxonal degeneration
dc.titleCharacterization of diabetic neuropathy progression in a mouse model of type 2 diabetes mellitus
dc.typeArticle

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
De Gregorio Contador D et al.pdf
Size:
2.96 MB
Format:
Adobe Portable Document Format
Description:
Texto completo
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services