Nanomechanical and Molecular Characterization of Aging in Dentinal Collagen

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dc.contributor.authorSchuh, Christina
dc.contributor.authorLeiva, Camila
dc.contributor.authorHuang, Sui
dc.contributor.authorBarrera, Nelson
dc.contributor.authorAguayo, Sebastian
dc.date.accessioned2023-01-09T19:35:21Z
dc.date.available2023-01-09T19:35:21Z
dc.date.issued2022
dc.description.abstractMethylglyoxal (MGO) is an important molecule derived from glucose metabolism with the capacity of attaching to collagen and generating advanced glycation end products (AGEs), which accumulate in tissues over time and are associated with aging and diseases. However, the accumulation of MGO-derived AGEs in dentin and their effect on the nanomechanical properties of dentinal collagen remain unknown. Thus, the aim of the present study was to quantify MGO-based AGEs in the organic matrix of human dentin as a function of age and associate these changes with alterations in the nanomechanical and ultrastructural properties of dentinal collagen. For this, 12 healthy teeth from <26-y-old and >50-y-old patients were collected and prepared to obtain crown and root dentin discs. Following demineralization, MGO-derived AGEs were quantified with a competitive ELISA. In addition, atomic force microscopy nanoindentation was utilized to measure changes in elastic modulus in peritubular and intertubular collagen fibrils. Finally, principal component analysis was carried out to determine aging profiles for crown and root dentin. Results showed an increased presence of MGO AGEs in the organic matrix of dentin in the >50-y-old specimens as compared with the <26-y-old specimens in crown and root. Furthermore, an increase in peritubular and intertubular collagen elasticity was observed in the >50-y-old group associated with ultrastructural changes in the organic matrix as determined by atomic force microscopy analysis. Furthermore, principal component analysis loading plots suggested different "aging profiles" in crown and root dentin, which could have important therapeutic implications in restorative and adhesive dentistry approaches. Overall, these results demonstrate that the organic matrix of human dentin undergoes aging-related changes due to MGO-derived AGEs with important changes in the nanomechanical behavior of collagen that may affect diagnostic and restorative procedures in older people.
dc.description.versionVersión publicada
dc.identifier.citationSchuh CMAP, Leiva-Sabadini C, Huang S, Barrera NP, Bozec L, Aguayo S. Nanomechanical and Molecular Characterization of Aging in Dentinal Collagen. J Dent Res. 2022 Jul;101(7):840-847. doi:10.1177/00220345211072484
dc.identifier.urihttps://doi.org/10.1177/00220345211072484
dc.identifier.urihttps://repositorio.udd.cl/handle/11447/6910
dc.language.isoen
dc.subjectAdvanced glycation end products
dc.subjectAtomic force microscopy
dc.subjectDental caries
dc.subjectDentin
dc.subjectEnzyme linked immunosorbent assay
dc.subjectPrincipal component analysis
dc.titleNanomechanical and Molecular Characterization of Aging in Dentinal Collagen
dc.typeArticle
dcterms.sourceJournal of dental research

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