Type I collagen hydrogels as a delivery matrix for royal jelly derived extracellular vesicles

Simple item page
dc.contributor.authorRamírez, Orlando J.
dc.contributor.authorAlvarez, Simón
dc.contributor.authorContreras-Kallens, Pamina
dc.contributor.authorBarrera, Nelson P.
dc.contributor.authorAguayo, Sebastian
dc.contributor.authorSchuh, Christina
dc.date.accessioned2021-07-12T16:39:45Z
dc.date.available2021-07-12T16:39:45Z
dc.date.issued2020
dc.description.abstractThroughout the last decade, extracellular vesicles (EVs) have become increasingly popular in several areas of regenerative medicine. Recently, Apis mellifera royal jelly EVs (RJ EVs) were shown to display favorable wound healing properties such as stimulation of mesenchymal stem cell migration and inhibition of staphylococcal biofilms. However, the sustained and effective local delivery of EVs in nonsystemic approaches – such as patches for chronic cutaneous wounds – remains an important challenge for the development of novel EV-based wound healing therapies. Therefore, the present study aimed to assess the suitability of type I collagen -a well-established biomaterial for wound healing – as a continuous delivery matrix. RJ EVs were integrated into collagen gels at different concentrations, where gels containing 2mg/ml collagen were found to display the most stable release kinetics. Functionality of released RJ EVs was confirmed by assessing fibroblast EV uptake and migration in a wound healing assay. We could demonstrate reliable EV uptake into fibroblasts with a sustained promigratory effect for up to 7 d. Integrating fibroblasts into the RJ EV-containing collagen gel increased the contractile capacity of these cells, confirming availability of RJ EVs to fibroblasts within the collagen gel. Furthermore, EVs released from collagen gels were found to inhibit Staphylococcus aureus ATCC 29213 biofilm formation. Overall, our results suggest that type I collagen could be utilized as a reliable, reproducible release system to deliver functional RJ EVs for wound healing therapies.es
dc.format.extent12 p.es
dc.identifier.citationDrug Delivery 2020, VOL. 27, NO. 1, 1308–1318es
dc.identifier.urihttps://doi.org/10.1080/10717544.2020.1818880es
dc.identifier.urihttp://hdl.handle.net/11447/4148
dc.language.isoenes
dc.subjectWound healinges
dc.subjectApis melliferaes
dc.subjectRegenerative medicinees
dc.subjectDrug deliveryes
dc.subjectExtracellular vesicle deliveryes
dc.titleType I collagen hydrogels as a delivery matrix for royal jelly derived extracellular vesicleses
dc.typeArticlees

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Type I collagen hydrogels as a delivery matrix for royal jelly derived extracellular vesicles.pdf
Size:
3.27 MB
Format:
Adobe Portable Document Format
Description:
Texto completo
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services