Regenerative Potential of Mesenchymal Stromal Cells: Age-Related Changes

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dc.contributor.authorBruna, Flavia
dc.contributor.authorContador, David
dc.contributor.authorConget, Paulette
dc.contributor.authorErranz, Benjamín
dc.contributor.authorSossa, Claudia
dc.contributor.authorArango-Rodríguez, Martha
dc.date.accessioned2017-01-04T17:24:37Z
dc.date.available2017-01-04T17:24:37Z
dc.date.issued2016
dc.descriptionCentro de Medicina Regenerativa
dc.description.abstractPreclinical and clinical studies have shown that a therapeutic effect results from mesenchymal stromal cells (MSCs) transplant. No systematic information is currently available regarding whether donor age modifies MSC regenerative potential on cutaneous wound healing. Here, we evaluate whether donor age influences this potential. Two different doses of bone marrow MSCs (BM-MSCs) from young, adult, or old mouse donors or two doses of their acellular derivatives mesenchymal stromal cells (acd-MSCs) were intradermally injected around wounds in the midline of C57BL/6 mice. Every two days, wound healing was macroscopically assessed (wound closure) and microscopically assessed (reepithelialization, dermal-epidermal junction, skin appendage regeneration, granulation tissue, leukocyte infiltration, and density dermal collagen fibers) after 12 days from MSC transplant. Significant differences in the wound closure kinetic, quality, and healing of skin regenerated were observed in lesions which received BM-MSCs from different ages or their acd-MSCs compared to lesions which received vehicle. Nevertheless, our data shows that adult’s BM-MSCs or their acd-MSCs were the most efficient for recovery of most parameters analyzed. Our data suggest that MSC efficacy was negatively affected by donor age, where the treatment with adult’s BM-MSCs or their acd-MSCs in cutaneous wound promotes a better tissue repair/regeneration. This is due to their paracrine factors secretion
dc.identifier.citationStem Cells International, March 2016, vol.2016, n°2016
dc.identifier.urihttp://hdl.handle.net/11447/922
dc.identifier.urihttp://dx.doi.org/10.1155/2016/1461648
dc.language.isoen_US
dc.publisherHindawi Publishing Corporation
dc.subjectStem cells
dc.subjectBone marrow
dc.subjectDonor age
dc.subjectDiabetic wounds
dc.subjectAdipose
dc.subjectMurine
dc.subjectRat
dc.subjectSkin
dc.titleRegenerative Potential of Mesenchymal Stromal Cells: Age-Related Changes
dc.typeArtículo

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