Opening of pannexin- and con nexin-based channels increases the excitability of nodose ganglion sensory neurons

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dc.contributor.authorRetamal, Mauricio
dc.contributor.authorAlcayaga, Julio
dc.contributor.authorVerdugo, Christian
dc.contributor.authorBultynck, Geert
dc.contributor.authorLeybaert, Luc
dc.contributor.authorSáez, Pablo
dc.contributor.authorFernández, Ricardo
dc.contributor.authorLeón, Luis
dc.contributor.authorSáez, Juan
dc.date.accessioned2017-03-07T15:06:13Z
dc.date.available2017-03-07T15:06:13Z
dc.date.issued2014
dc.descriptionCentro de Fisiología Celular e Integrativa
dc.description.abstractSatellite glial cells (SGCs) are the main glia in sensory ganglia. They surround neuronal bodies and form a cap that prevents the formation of chemical or electrical synapses between neighboring neurons. SGCs have been suggested to establish bidirectional paracrine communication with sensory neurons. However, the molecular mechanism involved in this cellular communication is unknown. In the central nervous system (CNS), astrocytes present connexin43 (Cx43) hemichannels and pannexin1 (Panx1) channels, and the opening of these channels allows the release of signal molecules, such as ATP and glutamate. We propose that these channels could play a role in glia-neuron communication in sensory ganglia. Therefore, we studied the expression and function of Cx43 and Panx1 in rat and mouse nodose-petrosal-jugular complexes (NPJcs) using confocal immunofluorescence, molecular and electrophysiological techniques. Cx43 and Panx1 were detected in SGCs and in sensory neurons, respectively. In the rat and mouse, the electrical activity of vagal nerve increased significantly after nodose neurons were exposed to a Ca2+/Mg2+-free solution, a condition that increases the open probability of Cx hemichannels. This response was partially mimicked by a cell-permeable peptide corresponding to the last 10 amino acids of Cx43 (TAT-Cx43CT). Enhanced neuronal activity was reduced by Cx hemichannel, Panx1 channel and P2X7 receptor blockers. Moreover, the role of Panx1 was confirmed in NPJc, because in those from Panx1 knockout mice showed a reduced increase of neuronal activity induced by Ca2+/Mg2+-free extracellular conditions. The data suggest that Cx hemichannels and Panx channels serve as paracrine communication pathways between SGCs and neurons by modulating the excitability of sensory neurons.
dc.format.extent12
dc.identifier.citationFrontiers in Cellular Neuroscience, 2014, 8: 158
dc.identifier.urihttp://hdl.handle.net/11447/1006
dc.identifier.urihttp://dx.doi.org/10.3389/fncel.2014.00158
dc.language.isoen_US
dc.publisherFrontiers Research Foundation
dc.subjectglial satellite cells
dc.subjectconnexon
dc.subjectperipheral glial cells
dc.subjectsensory ganglia
dc.subjectnodose ganglia
dc.titleOpening of pannexin- and con nexin-based channels increases the excitability of nodose ganglion sensory neurons
dc.typeArtículo

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