Rab24 interacts with the Rab7/Rab interacting lysosomal protein complex to regulate endosomal degradation.

Simple item page
dc.contributor.authorAmaya, Celina
dc.contributor.authorMilitello, Rodrigo
dc.contributor.authorCalligaris, Sebastian
dc.contributor.authorColombo, Maria
dc.date.accessioned2017-08-16T12:36:46Z
dc.date.available2017-08-16T12:36:46Z
dc.date.issued2016
dc.description.abstractEndocytosis is a multistep process engaged in extracellular molecules internalization. Several proteins including the Rab GTPases family coordinate the endocytic pathway. The small GTPase Rab7 is present in late endosome (LE) compartments being a marker of endosome maturation. The Rab interacting lysosomal protein (RILP) is a downstream effector of Rab7 that recruits the functional dynein/dynactin motor complex to late compartments. In the present study, we have found Rab24 as a component of the endosome-lysosome degradative pathway. Rab24 is an atypical protein of the Rab GTPase family, which has been attributed a function in vesicle trafficking and autophagosome maturation. Using a model of transiently expressed proteins in K562 cells, we found that Rab24 co-localizes in vesicular structures labeled with Rab7 and LAMP1. Moreover, using a dominant negative mutant of Rab24 or a siRNA-Rab24 we showed that the distribution of Rab7 in vesicles depends on a functional Rab24 to allow DQ-BSA protein degradation. Additionally, by immunoprecipitation and pull down assays, we have demonstrated that Rab24 interacts with Rab7 and RILP. Interestingly, overexpression of the Vps41 subunit from the homotypic fusion and protein-sorting (HOPS) complex hampered the co-localization of Rab24 with RILP or with the lysosomal GTPase Arl8b, suggesting that Vps41 would affect the Rab24/RILP association. In summary, our data strongly support the hypothesis that Rab24 forms a complex with Rab7 and RILP on the membranes of late compartments. Our work provides new insights into the molecular function of Rab24 in the last steps of the endosomal degradative pathway.
dc.format.extent16
dc.identifier.citationAmaya C, Militello RD, Calligaris SD, Colombo MI. Rab24 interacts with the Rab7/Rab interacting lysosomal protein complex to regulate endosomal degradation. Traffic. 2016 Nov;17(11):1181-1196.
dc.identifier.urihttp://hdl.handle.net/11447/1575
dc.identifier.urihttp://dx.doi.org/10.1111/tra.12431
dc.language.isoen_US
dc.publisherWiley
dc.subjectArl8b
dc.subjectendocytic pathway
dc.subjectlate endosomes
dc.subjectlysosomes
dc.subjectRab24
dc.subjectRab7
dc.subjectRILP
dc.subjectVps41
dc.titleRab24 interacts with the Rab7/Rab interacting lysosomal protein complex to regulate endosomal degradation.
dc.typeArtículo

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Rab24 interacts with the Rab7Rab interacting lysosomal protein complex to regulate endosomal degradation.pdf
Size:
24.14 MB
Format:
Adobe Portable Document Format
Description:
Texto completo
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services