Concordance analysis of ALK gene fusion detection methods in patients with Non– Small-Cell Lung Cancer from Chile, Brazil, and Peru

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dc.contributor.authorSepúlveda-Hermosilla, Gonzalo
dc.contributor.authorFreire, Matías
dc.contributor.authorBlanco, Alejandro
dc.contributor.authorCáceres, Javier
dc.contributor.authorLizana, Rodrigo
dc.contributor.authorRamos, Liliana
dc.contributor.authorAssar, Rodrigo
dc.contributor.authorAmpuero, Diego
dc.contributor.authorAren, Osvaldo
dc.contributor.authorChernilo, Sara
dc.contributor.authorSpencer, María Loreto
dc.contributor.authorBernal, Giuliano
dc.contributor.authorFlores, Jacqueline
dc.contributor.authorRasse, Germán
dc.contributor.authorSánchez, Carolina
dc.contributor.authorMarcelain, Katherine
dc.contributor.authorRivas, Solange
dc.contributor.authorPereira Branco, Gabriela
dc.contributor.authorGalli de Amorim, María
dc.contributor.authorNoronha Nunes, Diana
dc.contributor.authorDias-Neto, Emmanuel
dc.contributor.authorFreitas, Helano C.
dc.contributor.authorFernández, Cristina
dc.contributor.authorPérez, Paola
dc.contributor.authorNIRVANA team
dc.contributor.authorArmisén, Ricardo
dc.date.accessioned2021-08-03T14:02:10Z
dc.date.available2021-08-03T14:02:10Z
dc.date.issued2021-06
dc.description.abstractAbout 4 to 7 % of the Non-small cell lung cancer patients have ALK rearrangements and specific target therapies improve patients’ outcomes significantly. ALK gene fusions are detected by immunohistochemistry (IHC) or Fluorescent in situ Hybridization (FISH) as gold standards in South America. Next Generation Sequencing (NGS) based assays are a reliable alternative, able to perform simultaneous detection of multiple events from a single sample. We analyzed 4,240 Non-small cell lung cancer samples collected in 37 hospitals from Chile, Brazil, and Peru; where ALK rearrangements were determined as part of their standard of care (SofC) using either IHC or FISH. A subset of 1450 samples was sequenced with the Oncomine Focus Assay (OFA), and the concordance with the SofC tests was measured. An orthogonal analysis was performed using a qPCR EML4-ALK fusion detection kit. ALK fusion prevalence is very similar for Chile (3.67%, N=2142), Brazil (4.05%, N=1013) and Peru (4.59%, N=675). Whereas a comparison between OFA and SofC assays showed similar sensitivity, OFA had significantly higher specificity and higher positive predictive value, which opens new opportunities for a more specific determination of ALK gene rearrangements.es
dc.format.extent36 p.es
dc.identifier.citationThe Journal of Molecular Diagnostics, 2021 jun, 36 p.es
dc.identifier.urihttps://doi.org/10.1016/j.jmoldx.2021.05.018es
dc.identifier.urihttp://hdl.handle.net/11447/4215
dc.language.isoenes
dc.subjectLung canceres
dc.subjectALKes
dc.titleConcordance analysis of ALK gene fusion detection methods in patients with Non– Small-Cell Lung Cancer from Chile, Brazil, and Perues
dc.typeArticlees

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