Chronic hypoxia upregulates adenosine 2a receptor expression in chromaffin cells via hypoxia inducible factor-2a: Role in modulating secretion

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dc.contributor.authorBrown, Stephen T.
dc.contributor.authorReyes, Edison
dc.contributor.authorNurse, Colin A.
dc.date.accessioned2021-09-14T02:19:51Z
dc.date.available2021-09-14T02:19:51Z
dc.date.issued2011
dc.description.abstractCatecholamine (CAT) release from chromaffin tissue plays an essential role in the fetus which develops in a low O2 environment (hypoxia). To address molecular mechanisms regulating CAT secretion in low O2, we exposed a fetal chromaffin-derived cell line (MAH cells) to chronic hypoxia (CHox; 2% O2, 24 h) and assessed gene expression using microarrays, quantitative RT-PCR, and western blot. CHox caused a dramatic 12 upregulation of adenosine A2a receptor (A2aR) mRNA, an effect critically dependent upon hypoxia-inducible factor (HIF)-2a which bound the promoter of the A2aR gene. In amperometric studies, acute hypoxia and high K+ (30 mM) evoked quantal CAT secretion that was enhanced after CHox, and further potentiated during simultaneous A2aR activation by adenosine. A2aR activation also enhanced stimulus-induced rise in intracellular Ca2+ in control, but not HIF-2a-deficient, MAH cells. Thus, A2aR, adenosine, and HIF-2a are key contributors to the potentiation of CAT secretion in developing chromaffin cells during chronic hypoxia.es
dc.identifier.citationBiochemical and Biophysical Research Communications, 2011, 412: 466–472es
dc.identifier.urihttp://dx.doi.org/10.1016/j.bbrc.2011.07.122es
dc.identifier.urihttp://hdl.handle.net/11447/4620
dc.language.isoenes
dc.subjectHypoxiaes
dc.subjectHIF-2aes
dc.subjectChromaffin-derived MAH cellses
dc.subjectCatecholamineses
dc.subjectAdenosinees
dc.subjectAmperometryes
dc.subjectIntracellular Ca2+es
dc.titleChronic hypoxia upregulates adenosine 2a receptor expression in chromaffin cells via hypoxia inducible factor-2a: Role in modulating secretiones
dc.typeArticlees

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