Functional hemichannels formed by human connexin 26 expressed in bacteria

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dc.contributor.authorFiori, Mariana
dc.contributor.authorKrishnan, Srinivasan
dc.contributor.authorCortés, Marien
dc.contributor.authorRetamal, Mauricio
dc.contributor.authorReuss, Luis
dc.contributor.authorAltenberg, Guillermo
dc.contributor.authorCuello, Luis
dc.date.accessioned2016-05-23T12:58:41Z
dc.date.available2016-05-23T12:58:41Z
dc.date.issued2015
dc.descriptionCentro de Fisiología Celular e Integrativa
dc.description.abstractGap-junction channels (GJCs) communicate the cytoplasm of adjacent cells and are formed by head-to-head association of two hemichannels (HCs), one from each of the neighbouring cells. GJCs mediate electrical and chemical communication between cells, whereas undocked HCs participate in paracrine signalling because of their permeability to molecules such as ATP. Sustained opening of HCs under pathological conditions results in water and solute fluxes that cannot be compensated by membrane transport and therefore lead to cell damage. Mutations of Cx26 (connexin 26) are the most frequent cause of genetic deafness and it is therefore important to understand the structure-function relationship of wild-type and deafness-associated mutants. Currently available connexin HC expression systems severely limit the pace of structural studies and there is no simple high-throughput HC functional assay. The Escherichia coli-based expression system presented in the present study yields milligram amounts of purified Cx26 HCs suitable for functional and structural studies. We also show evidence of functional activity of recombinant Cx26 HCs in intact bacteria using a new growth complementation assay. The E. coli-based expression system has high potential for structural studies and high-throughput functional screening of HCs.
dc.identifier.citationFiori MC, Krishnan S, Cortes DM, Retamal MA, Reuss L, Altenberg GA, Cuello LG. Functional hemichannels formed by human connexin 26 expressed in bacteria. Biosci Rep. 2015 Mar 18;35(2):e00177
dc.identifier.urihttp://hdl.handle.net/11447/303
dc.identifier.urihttp://dx.doi.org/10.1042/BSR20140089
dc.language.isoen_US
dc.publisher© 2015 The Author(s) CC-BY Licence
dc.subjectConnexin 26
dc.subjectEscherichia coli,genetics
dc.subjectRecombinant proteins
dc.titleFunctional hemichannels formed by human connexin 26 expressed in bacteria
dc.typeArtículo

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