Comparison of VSV Pseudovirus and Focus Reduction Neutralization Assays for Measurement of Anti-Andes orthohantavirus Neutralizing Antibodies in Patient Samples

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dc.contributor.authorVial, Cecilia
dc.contributor.authorWhitaker, Annalis
dc.contributor.authorWilhelm, Jan
dc.contributor.authorOvalle, Jimena
dc.contributor.authorPerez, Ruth
dc.contributor.authorValdivieso, Francisca
dc.contributor.authorFerres, Marcela
dc.contributor.authorMartínez-Valdebenito, Constanza
dc.contributor.authorEisenhauer, Philip
dc.contributor.authorMertz, Gregory J.
dc.contributor.authorHooper, Jay W.
dc.contributor.authorBotten, Jason W.
dc.contributor.authorVial, Pablo
dc.date.accessioned2021-07-21T17:38:41Z
dc.date.available2021-07-21T17:38:41Z
dc.date.issued2020-09
dc.description.abstractAndes orthohantavirus (ANDV) is the etiologic agent of hantavirus cardiopulmonary syndrome (HCPS), which has a case fatality rate around 35%, with no effective treatment or vaccine available. ANDV neutralizing antibody (NAb) measurements are important for the evaluation of the immune response following infection, vaccination, or passive administration of investigational monoclonal or polyclonal antibodies. The standard assay for NAb measurement is a focus reduction neutralization test (FRNT) featuring live ANDV and must be completed under biosafety level (BSL)-3 conditions. In this study, we compared neutralization assays featuring infectious ANDV or vesicular stomatitis virus (VSV) pseudovirions decorated with ANDV glycoproteins for their ability to measure anti-ANDV NAbs from patient samples. Our studies demonstrate that VSV pseudovirions effectively measure NAb from clinical samples and have greater sensitivity compared to FRNT with live ANDV. Importantly, the pseudovirus assay requires less labor and sample materials and can be conducted at BSL-2.es
dc.identifier.citationFrontiers in Cellular and infection Microbiology, 2020 sep, vol.10:444es
dc.identifier.urihttps://doi.org/10.3389/fcimb.2020.00444es
dc.identifier.urihttp://hdl.handle.net/11447/4189
dc.language.isoenes
dc.subjectAndes orthohantavirus (ANDV)es
dc.subjectHantavirus cardiopulmonary syndrome (HCPS)es
dc.subjectNeutralizing antibodieses
dc.subjectOrthohantaviruses
dc.subjectVesicular stomatitis virus (VSV) pseudoviruses
dc.titleComparison of VSV Pseudovirus and Focus Reduction Neutralization Assays for Measurement of Anti-Andes orthohantavirus Neutralizing Antibodies in Patient Sampleses
dc.typeArticlees

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