Tubulointerstitial injury and proximal tubule albumin transport in early diabetic nephropathy induced by type 1 diabetes mellitus

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dc.contributor.authorGiraud-Billoud, Maximiliano
dc.contributor.authorEzquer, Fernando
dc.contributor.authorBahamonde, Javiera
dc.contributor.authorEzquer, Marcelo
dc.date.accessioned2017-09-11T13:34:42Z
dc.date.available2017-09-11T13:34:42Z
dc.date.issued2017
dc.description.abstractA decrease in the tubular expression of albumin endocytic transporters megalin and cubilin has been associated with diabetic nephropathy, but there are no comprehensive studies to date relating early tubulointerstitial injury and the effect of the disease on both transporters in type 1 diabetes mellitus (T1DM). We used eight-weekold male C57BL/6 mice divided into two groups; one of them received the vehicle (control group), while the other received the vehicle + 200 mg/kg streptozotocin (T1DM). Ten weeks after the injection, we evaluated plasma insulin, enzymuria, urinary vitamin D-binding protein (VDBP), tubulointerstitial fibrosis and proximal tubule histology, markers of autophagy, and megalin and cubilin levels. We found a reduction in tubular protein reabsorption (albumin and VDBP as specific substances carried by both transporters) with increased tubulointerstitial injury, development of fibrosis, thickening of tubular basement membrane, and an increase in tubular cell metalloproteases. This was associated with a decrease in the renal expression of megalin and cubilin. We also observed an increase in the amount of cellular vesicles of the phagocytic system in the tubules, which could be linked to an alteration of normal intracellular trafficking of both receptors, thus affecting the normal function of transporters in early stages of diabetic nephropathy. In diabetic animals, the added effects of tubulointerstitial injury, the decreases in megalin and cubilin expression, and an altered intracellular trafficking of these receptors, seriously affect protein reabsorption.
dc.description.versionVersión Publicada
dc.format.extent12
dc.identifier.citationGiraud Billoud, Maximiliano German; Ezquer, Fernando; Bahamonde, Javiera; Ezquer, Marcelo; Tubulointerstitial injury and proximal tubule albumin transport in early diabetic nephropathy induced by type 1 diabetes mellitus; Instituto de Histología y Embriología “Dr. Mario H. Burgos”; Biocell; 41; 1; 1-2017; 1-12
dc.identifier.urihttp://hdl.handle.net/11447/1659
dc.language.isoen_US
dc.publisherSociedad Latinoamericana de Microscopía Electrónica y Centro Regional de Investigaciones Científicas y Tecnológicas
dc.sourceBiocell
dc.subjectAlbuminuria
dc.subjectMegalin
dc.subjectCubilin
dc.subjectAutophagy
dc.titleTubulointerstitial injury and proximal tubule albumin transport in early diabetic nephropathy induced by type 1 diabetes mellitus
dc.typeArtículo

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