A novel gene cluster allows preferential utilization of fucosylated milk oligosaccharides in Bifidobacterium longum subsp. longum SC596

Simple item page
dc.contributor.authorGarrido, Daniel
dc.contributor.authorRuiz, Santiago
dc.contributor.authorKirmiz, Nina
dc.contributor.authorDavis, Jasmine
dc.contributor.authorTotten, Sarah
dc.contributor.authorLemay, Danielle
dc.contributor.authorUgalde, Juan
dc.contributor.authorGerman, Bruce
dc.contributor.authorLebrilla, Carlito
dc.contributor.authorMills, David
dc.date.accessioned2018-01-04T13:50:34Z
dc.date.available2018-01-04T13:50:34Z
dc.date.issued2016
dc.description.abstractThe infant intestinal microbiota is often colonized by two subspecies of Bifidobacterium longum: subsp. infantis (B. infantis) and subsp. longum (B. longum). Competitive growth of B. infantis in the neonate intestine has been linked to the utilization of human milk oligosaccharides (HMO). However, little is known how B. longum consumes HMO. In this study, infant-borne B. longum strains exhibited varying HMO growth phenotypes. While all strains efficiently utilized lacto-N-tetraose, certain strains additionally metabolized fucosylated HMO. B. longum SC596 grew vigorously on HMO, and glycoprofiling revealed a preference for consumption of fucosylated HMO. Transcriptomes of SC596 during early-stage growth on HMO were more similar to growth on fucosyllactose, transiting later to a pattern similar to growth on neutral HMO. B. longum SC596 contains a novel gene cluster devoted to the utilization of fucosylated HMO, including genes for import of fucosylated molecules, fucose metabolism and two α-fucosidases. This cluster showed a modular induction during early growth on HMO and fucosyllactose. This work clarifies the genomic and physiological variation of infant-borne B. longum to HMO consumption, which resembles B. infantis. The capability to preferentially consume fucosylated HMO suggests a competitive advantage for these unique B. longum strains in the breast-fed infant gut.
dc.format.extent18
dc.identifier.citationGarrido D, Ruiz-Moyano S, Kirmiz N, Davis JC, Totten SM, Lemay DG, Ugalde JA, German JB, Lebrilla CB, Mills DA. A novel gene cluster allows preferential utilization of fucosylated milk oligosaccharides in Bifidobacterium longum subsp. longum SC596. Sci Rep. 2016 Oct 19;6:35045
dc.identifier.urihttp://hdl.handle.net/11447/1859
dc.identifier.urihttp://dx.doi.org/10.1038/srep35045
dc.language.isoen_US
dc.publisherNature Publishing Group
dc.subjectApplied microbiology
dc.subjectBacterial genes
dc.subjectGlycobiology
dc.subjectTranscriptomics
dc.titleA novel gene cluster allows preferential utilization of fucosylated milk oligosaccharides in Bifidobacterium longum subsp. longum SC596
dc.typeArtículo

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
A novel gene cluster allows preferential utilization of fucosylated milk oligosaccharides in Bifidobacterium longum subsp. longum SC596__completo.pdf
Size:
1.89 MB
Format:
Adobe Portable Document Format
Description:
Texto completo
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services