Photochemically induced fluorescence coupled to second-order multivariate calibration as analytical tool for determining imidacloprid in honeybees

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dc.contributor.authorJeria, Yanara
dc.contributor.authorBazaes, Aliosha
dc.contributor.authorBaez, Maria E
dc.contributor.authorEspinoza, Jeanette
dc.contributor.authorMartinez, Jessica
dc.contributor.authorFuentes, Edwar
dc.date.accessioned2017-09-11T15:23:26Z
dc.date.available2017-09-11T15:23:26Z
dc.date.issued2017
dc.description.abstractThis paper presents a method for the determination of imidacloprid in honeybees based on the measurement of excitation-emission spectra of photo-induced fluorescence (PIF-EEMs) associated to unfolded partial least squares coupled to residual bilinearization (U-PLS/RBL) algorithm. As a preliminary step, matrix solid phase dispersion (MSPD) using C18 as dispersant, combined on-line with a solid phase extraction (SPE) clean-up on graphitized carbon-amino propyl silica phase was applied to diminish the interferences presents in samples. A previous study on the photochemical induction of fluorescence of imidacloprid in presence of bee matrix was included. The second order advantage achieved with RBL permitted the determination of imidacloprid in the presence of interferences present in samples (unexpected compounds of bees), which also shows photo-induced or native fluorescence. The LOD was 20 µg kg−1 (2.5 ng per bee; four bees treated), which is suitable for detecting imidacloprid at the oral LD50 for the insect. The predicted U-PLS/RBL concentrations compared favorably with those measured using high-performance liquid chromatography with diode array detection. The PIF-EEMs coupled to U-PLS/RBL was applied for the determination of imidacloprid in honeybees collected in field hives. The work demonstrates the feasibility of the determination of imidacloprid in a highly complex sample matrix as bee through photochemically induced fluorescence spectroscopy coupled to multivariate calibration.
dc.description.versionVersión Publicada
dc.format.extent7
dc.identifier.citationJeria, Y., Bazaes, A., Báez, M., Espinoza, J., Martínez, J. y Fuentes, E. (2017).Photochemically induced fluorescence coupled to second-order multivariate calibration as analytical tool for determining imidacloprid in honeybees. Disponible en https://repositorio.uchile.cl/handle/2250/158569
dc.identifier.urihttp://hdl.handle.net/11447/1664
dc.identifier.urihttp://dx.doi.org/10.1016/j.chemolab.2016.11.001
dc.language.isoen_US
dc.publisherElsevier
dc.sourceChemometrics and Intelligent Laboratory Systems
dc.subjectImidacloprid
dc.subjectHoneybees
dc.subjectPhoto-induced fluorescence
dc.subjectMultivariate calibration
dc.titlePhotochemically induced fluorescence coupled to second-order multivariate calibration as analytical tool for determining imidacloprid in honeybees
dc.typeArtículo

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