Identification and characterization of miRNAs and lncRNAs of coho salmon (Oncorhynchus kisutch) in normal immune organs

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dc.contributor.authorLeiva, Francisco
dc.contributor.authorRojas-Herrera, Marcelo
dc.contributor.authorReyes, Daniela
dc.contributor.authorBravo, Scarleth
dc.contributor.authorGarcía, Killen
dc.contributor.authorMoya, Javier
dc.contributor.authorVidal, Rodrigo
dc.date.accessioned2020-03-09T15:48:15Z
dc.date.available2020-03-09T15:48:15Z
dc.date.issued2020
dc.descriptionbCentro de Genética y Genómica, ICIM
dc.description.abstractMicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs) are two relevant non-coding RNAs (ncRNAs) class. Oncorhynchus kisutch (coho salmon) is an important aquaculture pacific salmon species without report of miRNAs and a very limited register of lncRNAs. To gain knowledge about the interaction and discovery of miRNAs and lncRNAs in coho salmon we used high-throughput sequencing technology to sequence small and transcriptome libraries from three immune organs. A total of 163 mature miRNAs and 4,975 lncRNAs were discovered. The profiles of expression of both ncRNAs indicated that liver and head-kidney share relatively similar expression patterns. We identified 814 and 181 putative target sequences for 1048 lncRNAs and 47 miRNAs, respectively. The results obtained provide new information and enlarge our understanding of the diversities of ncRNAs in coho salmon.
dc.identifier.citationGenomics 112(1): 45-54 January 2020
dc.identifier.urihttp://hdl.handle.net/11447/3127
dc.identifier.urihttps://doi.org/10.1016/j.ygeno.2019.07.015
dc.language.isoen
dc.publisherElsevier Inc
dc.subjectLong non-coding RNA
dc.subjectMicroRNA
dc.subjectGene expression
dc.subjectTarget gene
dc.subjectOncorhynchus kisutch
dc.subjectHigh-throughput sequencing
dc.titleIdentification and characterization of miRNAs and lncRNAs of coho salmon (Oncorhynchus kisutch) in normal immune organs
dc.typePreprint

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