Transcriptomic Analysis of Mouse Cochlear Supporting Cell Maturation Reveals Large-Scale Changes in Notch Responsiveness Prior to the Onset of Hearing

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dc.contributor.authorMaass, Juan C
dc.contributor.authorGu, Rende
dc.contributor.authorCai, Tianian
dc.contributor.authorWan, Ying-Wooi
dc.contributor.authorCantellano, Silvia C
dc.contributor.authorAsprer, Joanna S T
dc.contributor.authorZhang, Hongyuan
dc.contributor.authorJen, Hsin-I
dc.contributor.authorEdlund, Renée K
dc.contributor.authorLiu, Zhandong
dc.contributor.authorGroves, Andrew K
dc.date.accessioned2021-12-21T20:20:26Z
dc.date.available2021-12-21T20:20:26Z
dc.date.issued2016
dc.description.abstractNeonatal mouse cochlear supporting cells have a limited ability to divide and trans-differentiate into hair cells, but this ability declines rapidly in the two weeks after birth. This decline is concomitant with the morphological and functional maturation of the organ of Corti prior to the onset of hearing. However, despite this association between maturation and loss of regenerative potential, little is known of the molecular changes that underlie these events. To identify these changes, we used RNA-seq to generate transcriptional profiles of purified cochlear supporting cells from 1- and 6-day-old mice. We found many significant changes in gene expression during this period, many of which were related to regulation of proliferation, differentiation of inner ear components and the maturation of the organ of Corti prior to the onset of hearing. One example of a change in regenerative potential of supporting cells is their robust production of hair cells in response to a blockade of the Notch signaling pathway at the time of birth, but a complete lack of response to such blockade just a few days later. By comparing our supporting cell transcriptomes to those of supporting cells cultured in the presence of Notch pathway inhibitors, we show that the transcriptional response to Notch blockade disappears almost completely in the first postnatal week. Our results offer some of the first molecular insights into the failure of hair cell regeneration in the mammalian cochlea.es
dc.identifier.citationMaass JC, Gu R, Cai T, Wan YW, Cantellano SC, Asprer JS, Zhang H, Jen HI, Edlund RK, Liu Z, Groves AK. Transcriptomic Analysis of Mouse Cochlear Supporting Cell Maturation Reveals Large-Scale Changes in Notch Responsiveness Prior to the Onset of Hearing. PLoS One. 2016 Dec 5;11(12):e0167286es
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0167286es
dc.identifier.urihttp://hdl.handle.net/11447/5263
dc.language.isoen_USes
dc.subjectHearinges
dc.subjectAnimalses
dc.subjectCochleaes
dc.subjectTranscription, Genetices
dc.titleTranscriptomic Analysis of Mouse Cochlear Supporting Cell Maturation Reveals Large-Scale Changes in Notch Responsiveness Prior to the Onset of Hearinges
dc.typeArticlees

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