Molecular method for the detection of Andes hantavirus infection: validation for clinical diagnostics

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dc.contributor.authorVial, Cecilia
dc.contributor.authorMartinez-Valdebenito, Constanza
dc.contributor.authorRios, Susana
dc.contributor.authorMartinez, Jessica
dc.contributor.authorVial, Pablo
dc.contributor.authorFerres, Marcela
dc.contributor.authorRivera, Juan
dc.contributor.authorPerez, Ruth
dc.contributor.authorValdivieso, Francisca
dc.date.accessioned2017-05-15T15:31:18Z
dc.date.available2017-05-15T15:31:18Z
dc.date.issued2016
dc.description.abstractHantavirus cardiopulmonary syndrome is a severe disease caused by exposure to New World hantaviruses. Early diagnosis is difficult due to the lack of specific initial symptoms. Antihantavirus antibodies are usually negative until late in the febrile prodrome or the beginning of cardiopulmonary phase, while Andes hantavirus (ANDV) RNA genome can be detected before symptoms onset. We analyzed the effectiveness of quantitative reverse transcription polymerase chain reaction (RT-qPCR) as a diagnostic tool detecting ANDV-Sout genome in peripheral blood cells from 78 confirmed hantavirus patients and 166 negative controls. Our results indicate that RT-qPCR had a low detection limit (~10 copies), with a specificity of 100% and a sensitivity of 94.9%. This suggests the potential for establishing RT-qPCR as the assay of choice for early diagnosis, promoting early effective care of patients, and improving other important aspects of ANDV infection management, such as compliance of biosafety recommendations for health personnel in order to avoid nosocomial transmission.
dc.format.extent14
dc.identifier.citationVial C, Martinez-Valdebenito C, Rios S, Martinez J, Vial PA, Ferres M, Rivera JC, Perez R, Valdivieso F. Molecular method for the detection of Andes hantavirus infection: validation for clinical diagnostics. Diagn Microbiol Infect Dis. 2016 Jan;84(1):36-39
dc.identifier.urihttp://hdl.handle.net/11447/1247
dc.identifier.urihttp://dx.doi.org/10.1016/j.diagmicrobio.2015.07.019
dc.language.isoen_US
dc.publisherElsevier
dc.subjectAndes virus
dc.subjectdiagnosis
dc.subjecthantavirus
dc.subjectRT-qPCR
dc.titleMolecular method for the detection of Andes hantavirus infection: validation for clinical diagnostics
dc.typeArtículo

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