RNA-DNA differences in human mitochondria restore ancestral form of 16S ribosomal RNA

Simple item page
dc.contributor.authorBar-Yaacov, Dan
dc.contributor.authorAvital, Gal
dc.contributor.authorLevin, Liron
dc.contributor.authorRichards, Allison L.
dc.contributor.authorHachen, Naomi
dc.contributor.authorRebolledo-Jaramillo, Boris
dc.contributor.authorNekrutenko, Anton
dc.contributor.authorZarivach, Raz
dc.contributor.authorMishmar, Dan
dc.date.accessioned2019-07-10T22:15:19Z
dc.date.available2019-07-10T22:15:19Z
dc.date.issued2013
dc.description.abstractRNA transcripts are generally identical to the underlying DNA sequences. Nevertheless, RNA-DNA differences (RDDs) were found in the nuclear human genome and in plants and animals but not in human mitochondria. Here, by deep sequencing of human mitochondrial DNA (mtDNA) and RNA, we identified three RDD sites at mtDNA positions 295 (C-to-U), 13710 (A-to-U, A-to-G), and 2617 (A-to-U, A-to-G). Position 2617, within the 16S rRNA, harbored the most prevalent RDDs (>30% A-to-U and similar to 15% A-to-G of the reads in all tested samples). The 2617 RDDs appeared already at the precursor polycistrone mitochondrial transcript. By using traditional Sanger sequencing, we identified the A-to-U RDD in six different cell lines and representative primates (Gorilla gorilla, Pongo pigmaeus, and Macaca mulatta), suggesting conservation of the mechanism generating such RDD. Phylogenetic analysis of more than 1700 vertebrate mtDNA sequences supported a thymine as the primate ancestral allele at position 2617, suggesting that the 2617 RDD recapitulates the ancestral 16S rRNA. Modeling U or G (the RDDs) at position 2617 stabilized the large ribosomal subunit structure in contrast to destabilization by an A (the pre-RDDs). Hence, these mitochondrial RDDs are likely functional.
dc.format.extent8 p.
dc.identifier.citationGenome Research, 2013, Vol.: 23, n° 11, pp. 1789-1796
dc.identifier.urihttp://hdl.handle.net/11447/2521
dc.identifier.urihttp://dx.doi.org/10.1101/gr.161265.113
dc.language.isoen
dc.subjectHeteroplasmy
dc.subjectAlignment
dc.subjectSubunit
dc.subjectModel
dc.titleRNA-DNA differences in human mitochondria restore ancestral form of 16S ribosomal RNA
dc.typeArticle

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
RNA-DNA differences in human mitochondria restore ancestral form of 16S ribosomal RNA.pdf
Size:
2.52 MB
Format:
Adobe Portable Document Format
Description:
Texto completo
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services