Evaluation of a novel antigen-based rapid detection test for the diagnosis of SARS-CoV-2 in respiratory samples

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dc.contributor.authorPorte, Lorena
dc.contributor.authorLegarraga, Paulette
dc.contributor.authorVollrath, Valeska
dc.contributor.authorAguilera, Ximena
dc.contributor.authorMunita, José
dc.contributor.authorAraos, Rafael
dc.contributor.authorPizarro, Gabriel
dc.contributor.authorVial, Pablo
dc.contributor.authorIruretagoyena, Mirentxu
dc.contributor.authorDittrich, Sabine
dc.contributor.authorWeitzel, Thomas
dc.date.accessioned2021-07-14T15:20:54Z
dc.date.available2021-07-14T15:20:54Z
dc.date.issued2020
dc.description.abstractObjectives: In the context of the coronavirus disease 2019 (COVID-19) pandemic, the development and validation of rapid and easy-to-perform diagnostic methods are of high priority. This study was performed to evaluate a novel rapid antigen detection test (RDT) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in respiratory samples. Methods: The fluorescence immunochromatographic SARS-CoV-2 antigen test (Bioeasy Biotechnology Co., Shenzhen, China) was evaluated using universal transport medium with nasopharyngeal (NP) and oropharyngeal (OP) swabs from suspected COVID-19 cases. Diagnostic accuracy was determined in comparison to SARS-CoV-2 real-time (RT)-PCR. Results: A total of 127 samples were included; 82 were RT-PCR-positive. The median patient age was 38 years, 53.5% were male, and 93.7% were from the first week after symptom onset. Overall sensitivity and specificity were 93.9% (95% confidence interval 86.5-97.4%) and 100% (95% confidence interval 92.1-100%), respectively, with a diagnostic accuracy of 96.1% and Kappa coefficient of 0.9. Sensitivity was significantly higher in samples with high viral loads. Conclusions: The RDT evaluated in this study showed a high sensitivity and specificity in samples mainly obtained during the first week of symptoms and with high viral loads, despite the use of a non-validated sample material. The assay has the potential to become an important tool for early diagnosis of SARS-CoV-2, particularly in situations with limited access to molecular methods.es
dc.format.extent6 p.es
dc.identifier.citationInternational Journal of Infectious Diseases. 2020 Oct;99:328-333es
dc.identifier.urihttps://doi.org/10.1016/j.ijid.2020.05.098es
dc.identifier.urihttp://hdl.handle.net/11447/4176
dc.language.isoenes
dc.sourceInternational Journal of Infectious Diseases
dc.subjectAntigenes
dc.subjectCOVID-19es
dc.subjectCoronaviruses
dc.subjectDiagnosises
dc.subjectRapid diagnostic testes
dc.subjectSARS-CoV-2es
dc.titleEvaluation of a novel antigen-based rapid detection test for the diagnosis of SARS-CoV-2 in respiratory sampleses
dc.typeArticlees

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