Urinary Extracellular Vesicles as a Source of NGAL for Diabetic Kidney Disease Evaluation in Children and Adolescents With Type 1 Diabetes Mellitus

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dc.contributor.authorUgarte, Francisca
dc.contributor.authorSantapau, Daniela
dc.contributor.authorGallardo, Vivian
dc.contributor.authorGarfias, Carolina
dc.contributor.authorYizmeyián, Anahí
dc.contributor.authorVillanueva, Soledad
dc.contributor.authorSepúlveda, Carolina
dc.contributor.authorRocco, Jocelyn
dc.contributor.authorPasten, Consuelo
dc.contributor.authorUrquidi, Cinthya
dc.contributor.authorCavada, Gabriel
dc.contributor.authorSan Martin, Pamela
dc.contributor.authorCano, Francisco
dc.contributor.authorIrarrázabal, Carlos E.
dc.date.accessioned2022-03-28T15:00:26Z
dc.date.available2022-03-28T15:00:26Z
dc.date.issued2021
dc.description.abstractBackground: Tubular damage has a role in Diabetic Kidney Disease (DKD). We evaluated the early tubulointerstitial damage biomarkers in type-1 Diabetes Mellitus (T1DM) pediatric participants and studied the correlation with classical DKD parameters. Methods: Thirty-four T1DM and fifteen healthy participants were enrolled. Clinical and biochemical parameters [Glomerular filtration Rate (GFR), microalbuminuria (MAU), albumin/creatinine ratio (ACR), and glycated hemoglobin A1c (HbA1c)] were evaluated. Neutrophil gelatinase-associated lipocalin (NGAL), Hypoxia-inducible Factor-1a (HIF-1a), and Nuclear Factor of Activated T-cells-5 (NFAT5) levels were studied in the supernatant (S) and the exosome-like extracellular vesicles (E) fraction from urine samples. Results: In the T1DM, 12% had MAU >20 mg/L, 6% ACR >30 mg/g, and 88% had eGFR >140 ml/min/1.72 m2 . NGAL in the S (NGAL-S) or E (NGAL-E) fraction was not detectable in the control. The NGAL-E was more frequent (p = 0.040) and higher (p = 0.002) than NGAL-S in T1DM. The T1DM participants with positive NGAL had higher age (p = 0.03), T1DM evolution (p = 0.03), and serum creatinine (p = 0.003) than negative NGAL. The NGAL-E correlated positively with tanner stage (p = 0.0036), the median levels of HbA1c before enrollment (p = 0.045) and was independent of ACR, MAU, and HbA1c at the enrollment. NFAT5 and HIF-1a levels were not detectable in T1DM or control. Conclusion: Urinary exosome-like extracellular vesicles could be a new source of early detection of tubular injury biomarkers of DKD in T1DM patients.es
dc.description.versionVersión Publicadaes
dc.identifier.citationUgarte F, Santapau D, Gallardo V, Garfias C, Yizmeyia´ n A, Villanueva S, Sepu´lveda C, Rocco J, Pasten C, Urquidi C, Cavada G, San Martin P, Cano F and Irarra´ zabal CE (2022) Urinary Extracellular Vesicles as a Source of NGAL for Diabetic Kidney Disease Evaluation in Children and Adolescents With Type 1 Diabetes Mellitus. Front. Endocrinol. 12:654269. doi: 10.3389/fendo.2021.654269es
dc.identifier.urihttps://doi.org/10.3389/fendo.2021.654269es
dc.identifier.urihttp://hdl.handle.net/11447/5821
dc.language.isoenes
dc.subjectDiabetic kidney diseasees
dc.subjectType 1 diabetes mellituses
dc.subjectChildrenes
dc.subjectNGALes
dc.subjectUrinary extracellular vesicleses
dc.titleUrinary Extracellular Vesicles as a Source of NGAL for Diabetic Kidney Disease Evaluation in Children and Adolescents With Type 1 Diabetes Mellituses
dc.typeArticlees
dcterms.sourceFrontiers in Endocrinologyes

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