Ski Is Required for Tri-Methylation of H3K9 in Major Satellite and for Repression of Pericentromeric Genes: Mmp3, Mmp10 and Mmp13, in Mouse Fibroblasts

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dc.contributor.authorCapelli, Claudio
dc.contributor.authorSepúlveda, Hugo
dc.contributor.authorRivas, Solange
dc.contributor.authorVíctor, Paola
dc.contributor.authorUrzúa, Ulises
dc.contributor.authorDonoso, Gerardo
dc.contributor.authorSagredo, Eduardo
dc.contributor.authorCarrero, David
dc.contributor.authorCasanova-Ortiz, Emmanuel
dc.contributor.authorSagredo, Alfredo
dc.contributor.authorGonzález, Marisel
dc.contributor.authorManterola, Marcia
dc.contributor.authorNardocci, Gino
dc.contributor.authorArmisén, Ricardo
dc.contributor.authorMontecino, Martín
dc.contributor.authorMarcelain, Katherine
dc.date.accessioned2021-01-14T16:12:01Z
dc.date.available2021-01-14T16:12:01Z
dc.date.issued2020
dc.descriptionCentro de Genética y Genómica - ICIMes
dc.description.abstractSeveral mechanisms directing a rapid transcriptional reactivation of genes immediately after mitosis have been described. However, little is known about the maintenance of repressive signals during mitosis. In this work, we address the role of Ski in the repression of gene expression during M/G1 transition in mouse embryonic fibroblasts (MEFs). We found that Ski localises as a distinct pair of dots at the pericentromeric region of mitotic chromosomes, and the absence of the protein is related to high acetylation and low tri-methylation of H3K9 in pericentromeric major satellite. Moreover, differential expression assays in early G1 cells showed that the presence of Ski is significantly associated with repression of genes localised nearby to pericentromeric DNA. In mitotic cells, chromatin immunoprecipitation assays confirmed the association of Ski to major satellite and the promoters of the most repressed genes: Mmp3, Mmp10 and Mmp13. These genes are at pericentromeric region of chromosome 9. In these promoters, the presence of Ski resulted in increased H3K9 tri-methylation levels. This Ski-dependent regulation is also observed during interphase. Consequently, Mmp activity is augmented in Ski −/− MEFs. Altogether, these data indicate that association of Ski with the pericentromeric region of chromosomes during mitosis is required to maintain the silencing bookmarks of underlying chromatin.es
dc.identifier.citationJMB 432(10):3222-3238, 2020es
dc.identifier.urihttps://doi.org/10.1016/j.jmb.2020.03.013es
dc.identifier.urihttp://hdl.handle.net/11447/3718
dc.language.isoenes
dc.publisherElsevier Ltd.es
dc.subjectSkies
dc.subjectMMPes
dc.subjectMitotic bookmarkinges
dc.subjectH3K9me3es
dc.subjectMaSates
dc.titleSki Is Required for Tri-Methylation of H3K9 in Major Satellite and for Repression of Pericentromeric Genes: Mmp3, Mmp10 and Mmp13, in Mouse Fibroblastses
dc.typeArticlees

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