Spatiotemporal differences in gene expression between motor and sensory autografts and their effect on femoral nerve regeneration in the rat

Simple item page
dc.contributor.authorHercher, David
dc.contributor.authorKerbl, Markus
dc.contributor.authorSchuh, Christina
dc.contributor.authorHeinzel, Johannes
dc.contributor.authorGal, László
dc.contributor.authorStainer, Michaela
dc.contributor.authorSchmidhammer, Robert
dc.contributor.authorHausner, Thomas
dc.contributor.authorRedl, Heinz
dc.contributor.authorNógrádi, Antal
dc.contributor.authorHacobian, Ara
dc.date.accessioned2019-11-26T21:53:43Z
dc.date.available2019-11-26T21:53:43Z
dc.date.issued2019
dc.description.abstractTo improve the outcome after autologous nerve grafting in the clinic, it is important to understand the limiting variables such as distinct phenotypes of motor and sensory Schwann cells. This study investigated the properties of phenotypically different autografts in a 6 mm femoral nerve defect model in the rat, where the respective femoral branches distally of the inguinal bifurcation served as homotopic, or heterotopic autografts. Axonal regeneration and target reinnervation was analyzed by gait analysis, electrophysiology, and wet muscle mass analysis. We evaluated regeneration-associated gene expression between 5 days and 10 weeks after repair, in the autografts as well as the proximal, and distal segments of the femoral nerve using qRT-PCR. Furthermore we investigated expression patterns of phenotypically pure ventral and dorsal roots. We identified highly significant differences in gene expression of a variety of regeneration-associated genes along the central – peripheral axis in healthy femoral nerves. Phenotypically mismatched grafting resulted in altered spatiotemporal expression of neurotrophic factor BDNF, GDNF receptor GFRα1, cell adhesion molecules Cadm3, Cadm4, L1CAM, and proliferation associated Ki67. Although significantly higher quadriceps muscle mass following homotopic nerve grafting was measured, we did not observe differences in gait analysis, and electrophysiological parameters between treatment paradigms. Our study provides evidence for phenotypic commitment of autologous nerve grafts after injury and gives a conclusive overview of temporal expression of several important regeneration-associated genes after repair with sensory or motor graft.
dc.format.extent19 p.
dc.identifier.citationFrontiers in cellular Neuroscience, 2019, 13(182)
dc.identifier.urihttp://hdl.handle.net/11447/2870
dc.language.isoen
dc.subjectFemoral nerve
dc.subjectSchwann cell
dc.subjectPhenotype
dc.subjectGene expression
dc.subjectNeurotrophic factor
dc.subjectCell adhesion molecule
dc.subjectPeripheral nerve regeneration
dc.titleSpatiotemporal differences in gene expression between motor and sensory autografts and their effect on femoral nerve regeneration in the rat
dc.typeArticle

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Hercher_Front_2019 - CHRISTINA SCHUH ..pdf
Size:
4.92 MB
Format:
Adobe Portable Document Format
Description:
Texto completo
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services