Proteomic surfaceome analysis of mesothelioma

Simple item page
dc.contributor.authorZiegler, Annemarie
dc.contributor.authorCerciello, Ferdinando
dc.contributor.authorBigosch, Colette
dc.contributor.authorBausch-Fluck, Damaris
dc.contributor.authorFelley-Bosco, Emanuela
dc.contributor.authorOssola, Reto
dc.contributor.authorSoltermann, Alex
dc.contributor.authorStahel, Rolf
dc.contributor.authorWollscheid, Bernd
dc.date.accessioned2021-10-04T14:29:54Z
dc.date.available2021-10-04T14:29:54Z
dc.date.issued2012
dc.description.abstractIdentification of new markers for malignant pleural mesothelioma (MPM) is a challenging clinical need. Here, we propose a quantitative proteomics primary screen of the cell surface exposed MPM Nglycoproteins, which provides the basis for the development of new protein-based diagnostic assays. Using the antibody-independent mass-spectrometry based cell surface capturing (CSC) technology, we specifically investigated the N-glycosylated surfaceome of MPM towards the identification of proteinmarker candidates discriminatory between MPM and lung adenocarcinoma (ADCA). Relative quantitative CSC analysis of MPM cell line ZL55 in comparison with ADCA cell line Calu-3 revealed a bird’s eye view of their respective surfaceomes.Ina secondary screenoffifteenMPMandsixADCA, weusedhighthroughput low density microarrays (LDAs) to verify specificity and sensitivity of nineteen N-glycoproteins overregulated in the surfaceome of MPM. This proteo-transcriptomic approach revealed thy-1/CD90 (THY1) and teneurin-2 (ODZ2) as protein-marker candidates for the discrimination of MPM from ADCA. Thy1/CD90 was further validated by immunohistochemistry on frozen tissue sections of MPM and ADCA samples. Together, we present a combined proteomic and transcriptomic approach enabling the relative quantitative identification and pre-clinical selection of new MPM marker candidates.es
dc.identifier.citationLung Cancer, 2012; 75:189-196es
dc.identifier.urihttp://dx.doi.org/10.1016/j.lungcan.2011.07.009es
dc.identifier.urihttp://hdl.handle.net/11447/4775
dc.language.isoenes
dc.subjectMesotheliomaes
dc.subjectSurfaceomees
dc.subjectCell surface capturing (CSC) technologyes
dc.subjectN-glycoproteinses
dc.subjectSurface markerses
dc.subjectCD90es
dc.subjectODZ2es
dc.titleProteomic surfaceome analysis of mesotheliomaes
dc.typeArticlees

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Proteomic surfaceome analysis of mesothelioma.pdf
Size:
1.57 MB
Format:
Adobe Portable Document Format
Description:
Texto completo
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services