Human renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma

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dc.contributor.authorBruna, Flavia
dc.contributor.authorCampo-Verde-Arbocco, Fiorella
dc.contributor.authorContador, David
dc.contributor.authorGómez, Silvina
dc.contributor.authorSantiano, Flavia
dc.contributor.authorSasso, Corina
dc.contributor.authorRomeo, Leonardo
dc.contributor.authorZyla, Leila
dc.contributor.authorLópez-Fontana, Constanza
dc.contributor.authorCalvo, Juan Carlos
dc.contributor.authorCarón, Rubén
dc.contributor.authorPistone-Creydt, Virginia
dc.date.accessioned2020-01-24T11:56:30Z
dc.date.available2020-01-24T11:56:30Z
dc.date.issued2019
dc.descriptionCentro de Medicina Regenerativa (ICIM)
dc.description.abstractTumor cells can interact with neighboring adipose tissue. We evaluated components present in human adipose explants from normal (hRAN) and kidney cancer (hRAT) tissue, and we evaluated the effects of conditioned media (CMs) from hRAN and hRAT on proliferation, adhesion and migration of tumor and nontumor human renal epithelial cell lines. In addition, we evaluated the expression of AdipoR1, ObR, CD44, vimentin, pERK and pPI3K on cell lines incubated with CMs. hRAN were obtained from healthy operated donors, and hRAT from patients who underwent a nephrectomy. hRAT showed increased levels of versican, leptin and ObR; and decreased levels of perilipin, adiponectin and AdipoR1, compared to hRAN. Cell lines showed a significant decrease in cell adhesion and increase in cell migration after incubation with hRAT-CMs vs. hRAN- or control-CMs. Surprisingly, HK-2, 786- O and ACHN cells showed a significant decrease in cell migration after incubation with hRAN-CMs vs. control-CMs. No difference in proliferation of cell lines was found after 24 or 48 h of treatment with CMs. AdipoR1 in ACHN and Caki-1 cells decreased significantly after incubation with hRAT-CMs vs. hRAN-CMs and control-CMs. ObR and CD44 increased in tumor line cells, and vimentin increased in non-tumor cells, after incubation with hRAT-CMs vs. hRAN-CMs and control-CMs. We observed an increase in the expression of pERK and pPI3K in HK-2, 786-O and ACHN, incubated with hRATCMs. In conclusion, results showed that adipose microenvironment can regulate the behavior of tumor and non tumor human renal epithelial cells.
dc.identifier.citationOncotarget, 2019, Vol. 10, (No. 52), pp: 5454-5467
dc.identifier.urihttp://hdl.handle.net/11447/3068
dc.language.isoen
dc.publisherImpact Journals, LLC
dc.subjectHuman renal adipose tissue
dc.subjectRenal epithelial cells
dc.subjectCancer
dc.subjectEpithelial-stromal interactions
dc.subjectMigration
dc.titleHuman renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma
dc.typeArticle

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