Modulatory Effect of Glycated Collagen on Oral Streptococcal Nanoadhesion

Simple item page
dc.contributor.authorBenso, B.
dc.contributor.authorNaulin, P.A.
dc.contributor.authorBarrera, N.P.
dc.contributor.authorBozec, L.
dc.contributor.authorAguayo, S.
dc.contributor.authorSchuh, Christina
dc.date.accessioned2021-08-05T15:23:59Z
dc.date.available2021-08-05T15:23:59Z
dc.date.issued2021
dc.description.abstractBiofilm-mediated oral diseases such as dental caries and periodontal disease remain highly prevalent in populations worldwide. Biofilm formation initiates with the attachment of primary colonizers onto surfaces, and in the context of caries, the adhesion of oral streptococcito dentinal collagen is crucial for biofilm progression. It is known that dentinal collagen suffers from glucose-associated crosslinking as a function of aging or disease; however, the effect of collagen crosslinking on the early adhesion and subsequent biofilm formation of relevant oral streptococci remains unknown. Therefore, the aim of this work was to determine the impact of collagen glycation on the initial adhesion of primary colonizers such as Streptococcus mutans UA159 and Streptococcus sanguinis SK 36, as well as its effect on the early stages of streptococcal biofilm formation in vitro. Type I collagen matrices were crosslinked with either glucose or methylglyoxal. Atomic force microscopy nanocharacterization revealed morphologic and mechanical changes within the collagen matrix as a function of crosslinking, such as a significantly increased elastic modulus in crosslinked fibrils. Increased nanoadhesion forces were observed for S. mutans on crosslinked collagen surfaces as compared with the control, and retraction curves obtained for both streptococcal strains demonstrated nanoscale unbinding behavior consistent with bacterial adhesin-substrate coupling. Overall, glucose-crosslinked substrates specifically promoted the initial adhesion, biofilm formation, and insoluble extracellular polysaccharide production of S. mutans, while methylglyoxal treatment reduced biofilm formation for both strains. Changes in the adhesion behavior and biofilm formation of oral streptococci as a function of collagen glycation could help explain the biofilm dysbiosis seen in older people and patients with diabetes. Further studies are necessary to determine the influence of collagen crosslinking on the balance between acidogenic and nonacidogenic streptococci to aid in the development of novel preventive and therapeutic treatment against dental caries in these patients.es
dc.identifier.citationJournal of Dental Research, 2021, Vol. 100(1) 82–89es
dc.identifier.urihttps://doi.org/10.1177/0022034520946320es
dc.identifier.urihttp://hdl.handle.net/11447/4233
dc.language.isoenes
dc.subjectAtomic force microscopyes
dc.subjectBacterial adhesiones
dc.subjectBiofilmses
dc.subjectAginges
dc.subjectDental carieses
dc.subjectStreptococcus mutanses
dc.titleModulatory Effect of Glycated Collagen on Oral Streptococcal Nanoadhesiones
dc.typeArticlees

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Modulatory Effect of Glycated Collagen on Oral Streptococcal Nanoadhesion.pdf
Size:
3.31 MB
Format:
Adobe Portable Document Format
Description:
Texto completo
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services