Analysis of FGF-dependent and FGF-independent pathways in otic placode induction

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dc.contributor.authorYang, Lu
dc.contributor.authorO’Neill, Paul
dc.contributor.authorMartin, Kareen
dc.contributor.authorMaass, Juan
dc.contributor.authorVassilev, Vassil
dc.contributor.authorLadher, Raj
dc.contributor.authorGroves, Andrew
dc.date.accessioned2017-04-13T14:02:15Z
dc.date.available2017-04-13T14:02:15Z
dc.date.issued2013
dc.description.abstractThe inner ear develops from a patch of thickened cranial ectoderm adjacent to the hindbrain called the otic placode. Studies in a number of vertebrate species suggest that the initial steps in induction of the otic placode are regulated by members of the Fibroblast Growth Factor (FGF) family, and that inhibition of FGF signaling can prevent otic placode formation. To better understand the genetic pathways activated by FGF signaling during otic placode induction, we performed microarray experiments to estimate the proportion of chicken otic placode genes that can be up-regulated by the FGF pathway in a simple culture model of otic placode induction. Surprisingly, we find that FGF is only sufficient to induce about 15% of chick otic placode-specific genes in our experimental system. However, pharmacological blockade of the FGF pathway in cultured chick embryos showed that although FGF signaling was not sufficient to induce the majority of otic placode-specific genes, it was still necessary for their expression in vivo. These inhibitor experiments further suggest that the early steps in otic placode induction regulated by FGF signaling occur through the MAP kinase pathway. Although our work suggests that FGF signaling is necessary for otic placode induction, it demonstrates that other unidentified signaling pathways are required to co-operate with FGF signaling to induce the full otic placode program.
dc.format.extent13
dc.identifier.citationPLoS One, 2013, 8(1): e55011
dc.identifier.urihttp://hdl.handle.net/11447/1143
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0055011
dc.language.isoen_US
dc.publisherPLoS
dc.subjectotic placode
dc.subjectFibroblast Growth Factor/FGF
dc.subjectmicroarray experiments
dc.subjectchick
dc.titleAnalysis of FGF-dependent and FGF-independent pathways in otic placode induction
dc.typeArtículo

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