The pore forming capacity of Sticholysin I in dipalmitoyl phosphatidyl vesicles is tuned by osmotic stress

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dc.contributor.authorAhumada, Manuel
dc.contributor.authorCalderon, Cristian
dc.contributor.authorLissi, E
dc.contributor.authorAlvarez, Carlos
dc.contributor.authorLanio, Maria
dc.date.accessioned2018-01-10T13:40:00Z
dc.date.available2018-01-10T13:40:00Z
dc.date.issued2017
dc.description.abstractThe osmotic condition modulates the properties of liposomes, particularly those related to their stability and response to external agents such as membrane-active proteins or peptides. In a previous work, we have demonstrated that an osmotic shock can increase, per se, water influx/efflux and the exit of the fluorophore calcein entrapped in the aqueous pool of dipalmitoylphosphatidylcholine (DPPC) and DPPC:sphingomyelin (SM) large unilamellar vesicles (LUVs), suggesting a loss of integrity of the liposome bilayer. In the present work, we have extended our study in order to assess how an osmotic imbalance prior to or synchronous with the addition of a recombinant variant of the pore-forming toxin sticholysin I (rSt I) modifies its pore forming capacity in DPPC and DPPC:SM (1:1) LUVs. Our results conclusively show the capacity of hypotonic gradients to improve the pore forming capacity of rSt I molecules, even in pure DPPC liposomes, rendering pore-formation less dependent on the presence of sphyngomyelin. In fact, non-active toxins in DPPC liposomes become active by a hypotonic imbalance in a similar way to those containing SM as a second component.
dc.description.versionVersión Publicada
dc.format.extent7
dc.identifier.citationAhumada M, Calderon C, Lissi E, Alvarez C, Lanio ME, Pazos F. The pore forming capacity of Sticholysin I in dipalmitoyl phosphatidyl vesicles is tuned by osmotic stress. Chem Phys Lipids. 2017 Mar;203:87-93. doi: 10.1016/j.chemphyslip.2016.12.005.
dc.identifier.urihttp://hdl.handle.net/11447/1878
dc.identifier.urihttp://dx.doi.org/10.1016/j.chemphyslip.2016.12.005
dc.language.isoen_US
dc.publisherElsevier
dc.sourceChemistry and Physics of Lipids
dc.subjectSticholysins
dc.subjectOsmotic shock
dc.subjectLiposomes
dc.subjectPermeability
dc.titleThe pore forming capacity of Sticholysin I in dipalmitoyl phosphatidyl vesicles is tuned by osmotic stress
dc.typeArtículo

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