Temperature dependence of bilayer properties in liposomes and the use of fluorescent probes as a tool to elucidate the permeation mechanism of hydrophilic solutes

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dc.contributor.authorAhumada, M
dc.contributor.authorCalderon, C
dc.contributor.authorLissie, A
dc.date.accessioned2017-12-28T18:22:24Z
dc.date.available2017-12-28T18:22:24Z
dc.date.issued2016
dc.description.abstractSolute transport across lipidic membranes is a fundamental process for both living organisms and drug delivery. In order to establish the mechanism of solute passive transport through lipidic membranes, we determined the effect of temperature upon the rate of glucose transport (as hydrophilic solute model) through dipalmitoylphosphatidylcholine large unilamellar liposomes and compare the results to those obtained for a variety of fluorescent probes (pyrene, PRODAN, diphenylhexatriene, diphenylhexatriene-TMA). All these probes, independent of their localization in the liposomes, report a monotonous change in the micro-properties sensed with temperature, with a maximum rate of change at the main transition temperature of the bilayer. These results contrast with those obtained for the rate of glucose influx, were it is observed a clear maximum rate of intake at the transition temperature. These contrasting results imply that the microviscosity (common factor for used probes) is not the property of the bilayer that controls the rate off solute transport which, at least in the vicinity of the main transition temperature, is regulated by formation of transient pores, particularly at the transition temperature (Tm). This would indicate that, at least near Tm, glucose and other hydrophilic solutes diffuses through transient pores formed in the bilayer. Interestingly, the presence of these pores does not affect the fluorescence characteristics of the probes.
dc.format.extent3
dc.identifier.citationElectronic Document Format(APA) AHUMADA, M, CALDERÓN, C, & LISSIE, A. (2016). TEMPERATURE DEPENDENCE OF BILAYER PROPERTIES IN LIPOSOMES AND THE USE OF FLUORESCENT PROBES AS A TOOL TO ELUCIDATE THE PERMEATION MECHANISM OF HYDROPHILIC SOLUTES. Journal of the Chilean Chemical Society, 61(3), 3052-3054.
dc.identifier.urihttp://hdl.handle.net/11447/1802
dc.identifier.urihttp://dx.doi.org/10.4067/S0717-97072016000300009
dc.language.isoen_US
dc.publisherSociedad Chilena de Química
dc.subjectliposome
dc.subjectpermeation
dc.subjectglucose
dc.subjectglucose oxidase
dc.subjectfluorescent probe
dc.titleTemperature dependence of bilayer properties in liposomes and the use of fluorescent probes as a tool to elucidate the permeation mechanism of hydrophilic solutes
dc.typeArtículo

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