Connexin46 Expression Enhances Cancer Stem Cell and Epithelial-to-Mesenchymal Transition Characteristics of Human Breast Cancer MCF-7 Cells

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dc.contributor.authorAcuña, Rodrigo
dc.contributor.authorVaras-Godoy, Manuel
dc.contributor.authorHerrera-Sepúlveda, Diego
dc.contributor.authorRetamal, Mauricio
dc.date.accessioned2021-11-25T19:15:44Z
dc.date.available2021-11-25T19:15:44Z
dc.date.issued2021
dc.description.abstractConnexins (Cxs) are a family of proteins that form two different types of ion channels: hemichannels and gap junction channels. These channels participate in cellular communication, enabling them to share information and act as a synchronized syncytium. This cellular communication has been considered a strong tumor suppressor, but it is now recognized that some type of Cxs can be pro-tumorigenic. For example, Cx46 expression is increased in human breast cancer samples and correlates with cancer stem cell (CSC) characteristics in human glioma. Thus, we explored whether Cx46 and glioma cells, can set up CSC and epithelial-to-mesenchymal transition (EMT) properties in a breast cancer cell line. To this end, we transfected MCF-7 cells with Cx46 attached to a green fluorescent protein (Cx46GFP), and we determined how its expression orchestrates both the gene-expression and functional changes associated with CSC and EMT. We observed that Cx46GFP increased Sox2, Nanog, and OCT4 mRNA levels associated with a high capacity to form monoclonal colonies and tumorspheres. Similarly, Cx46GFP increased the mRNA levels of n-cadherin, Vimentin, Snail and Zeb1 to a higher migratory and invasive capacity. Furthermore, Cx46GFP transfected in MCF-7 cells induced the release of higher amounts of VEGF, which promoted angiogenesis in HUVEC cells. We demonstrated for the first time that Cx46 modulates CSC and EMT properties in breast cancer cells and thus could be relevant in the design of future cancer therapieses
dc.identifier.citationAcuña, Rodrigo A., Manuel Varas-Godoy, Diego Herrera-Sepulveda, and Mauricio A. Retamal 2021. "Connexin46 Expression Enhances Cancer Stem Cell and Epithelial-to-Mesenchymal Transition Characteristics of Human Breast Cancer MCF-7 Cells" International Journal of Molecular Sciences 22, no. 22: 12604. https://doi.org/10.3390/ijms222212604es
dc.identifier.urihttps://www.mdpi.com/1422-0067/22/22/12604es
dc.identifier.urihttp://hdl.handle.net/11447/5109
dc.language.isoenes
dc.subjectConnexin46es
dc.subjectBreast canceres
dc.subjectCancer stem cellses
dc.subjectEpithelial-to-mesenchymal transitiones
dc.subjectStemnesses
dc.titleConnexin46 Expression Enhances Cancer Stem Cell and Epithelial-to-Mesenchymal Transition Characteristics of Human Breast Cancer MCF-7 Cellses
dcterms.typeArticlees

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