Frizzled-1 receptor regulates adult hippocampal neurogenesis

Simple item page
dc.contributor.authorMardones, Muriel
dc.contributor.authorAndaur, Gabriela
dc.contributor.authorVaras-Godoy, Manuel
dc.contributor.authorHenriquez, Jenny
dc.contributor.authorSalech, Felipe
dc.contributor.authorBehrens, María Isabel
dc.contributor.authorCouve, Andres
dc.contributor.authorInestrosa, Nibaldo
dc.contributor.authorVarela-Nallar, Lorena
dc.date.accessioned2017-08-09T19:48:24Z
dc.date.available2017-08-09T19:48:24Z
dc.date.issued2016
dc.description.abstractBACKGROUND: In the adult hippocampus new neurons are continuously generated from neural stem cells (NSCs) present at the subgranular zone of the dentate gyrus. This process is controlled by Wnt signaling, which plays a complex role in regulating multiple steps of neurogenesis including maintenance, proliferation and differentiation of progenitor cells and the development of newborn neurons. Differential effects of Wnt signaling during progression of neurogenesis could be mediated by cell-type specific expression of Wnt receptors. Here we studied the potential role of Frizzled-1 (FZD1) receptor in adult hippocampal neurogenesis. RESULTS: In the adult dentate gyrus, we determined that FZD1 is highly expressed in NSCs, neural progenitors and immature neurons. Accordingly, FZD1 is expressed in cultured adult hippocampal progenitors isolated from mouse brain. To evaluate the role of this receptor in vivo we targeted FZD1 in newborn cells using retroviral-mediated RNA interference. FZD1 knockdown resulted in a marked decrease in the differentiation of newborn cells into neurons and increased the generation of astrocytes, suggesting a regulatory role for the receptor in cell fate commitment. In addition, FZD1 knockdown induced an extended migration of adult-born neurons within the granule cell layer. However, no differences were observed in total dendritic length and dendritic arbor complexity between control and FZD1-deficient newborn neurons. CONCLUSIONS: Our results show that FZD1 regulates specific stages of adult hippocampal neurogenesis, being required for neuronal differentiation and positioning of newborn neurons into the granule cell layer, but not for morphological development of adult-born granule neurons.
dc.format.extent1
dc.identifier.citationMol Brain. 2016 Mar 15;9:29
dc.identifier.urihttp://hdl.handle.net/11447/1550
dc.identifier.urihttp://dx.doi.org/10.1186/s13041-016-0209-3
dc.language.isoen_US
dc.publisherBioMed Central
dc.subjectAdult neurogenesis
dc.subjectFrizzled
dc.subjectHippocampus
dc.subjectNeural progenitor cells
dc.subjectNeuronal differentiation
dc.subjectWnt signaling
dc.titleFrizzled-1 receptor regulates adult hippocampal neurogenesis
dc.typeArtículo

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Frizzled-1 receptor regulates adult hippocampal neurogenesis.pdf
Size:
2.26 MB
Format:
Adobe Portable Document Format
Description:
Texto completo
Download

Collections

Artículos Medicina y Ciencias de la Salud

Implementado por OpenGeek Services