Surfaces based on amino acid functionalized polyelectrolyte films towards active surfaces for enzyme immobilization

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dc.contributor.authorBriones, Ximena
dc.contributor.authorVillalobos, Valeria
dc.contributor.authorQueneau, Yves
dc.contributor.authorSilva Danna, Caroline
dc.contributor.authorMuñoz, Rodrigo
dc.contributor.authorRíos, Hernán E.
dc.contributor.authorPavez, Jorge
dc.contributor.authorPáez, Maritza
dc.contributor.authorCabrera, Ricardo
dc.contributor.authorTamayo, Laura
dc.contributor.authorUrzúa, Marcela D.
dc.date.accessioned2022-07-12T21:38:49Z
dc.date.available2022-07-12T21:38:49Z
dc.date.issued2019
dc.description.abstractSurface based on polyelectrolytes functionalized with amino acids onto amino-terminated solid surfaces of silicon wafers was prepared, with the purpose of evaluate the chemical functionality of the polyelectrolyte films in adsorption and catalytic activity of an enzyme. In this work, the adsorption of the enzyme glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides (LmG6PD) was studied as model. The polyelectrolytes were obtained from poly (maleic anhydride-alt-vinylpyrrolidone) [poly(MA-alt-VP)] and functionalized with amino acids of different hydropathy index: glutamine (Gln), tyrosine (Tyr) and methionine (Met). The polyelectrolytes were adsorbed onto the amino-terminated silicon wafer at pH 3.5 and 4.5 and at low and high ionic strength. At low ionic strength and pH 3.5, the largest quantity of adsorbed polyelectrolyte was on the films containing glutamine moiety as the most hydrophilic amino acid in the side chain of polymer chain (5.88 mg/m2), whereas at high ionic strength and pH 4.5, the lowest quantity was in films containing tyrosine moiety in the side chain (1.88 mg/m2). The films were characterized by ellipsometry, contact angle measurements and atomic force microscopy (AFM). The polyelectrolyte films showed a moderate degree of hydrophobicity, the methionine derivative being the most hydrophobic film. With the aim of evaluate the effect of the amino acid moieties on the ability of the surface to adsorb enzymes, we study the activity of the enzyme on these surfaces. We observed that the polarity of the side chain of the amino acid in the polyelectrolyte affected the quantity of LmG6PD adsorbed, as well as its specific activity, showing that films prepared from poly(MA-alt-VP) functionalized with Met provide the best enzymatic performance. The results obtained demonstrated that the surfaces prepared from polyelectrolytes functionalized with amino acids could be an attractive and simple platform for the immobilization of enzymes, which could be of interest for biocatalysis applicationses
dc.description.versionVersión Publicadaes
dc.identifier.citationXimena Briones, Valeria Villalobos, Yves Queneau, Caroline Silva Danna, Rodrigo Muñoz, Hernán E. Ríos, Jorge Pavez, Maritza Páez, Ricardo Cabrera, Laura Tamayo, Marcela D. Urzúa, Surfaces based on amino acid functionalized polyelectrolyte films towards active surfaces for enzyme immobilization, Materials Science and Engineering: C, Volume 104, 2019, 109938, ISSN 0928-4931, https://doi.org/10.1016/j.msec.2019.109938.es
dc.identifier.urihttps://doi.org/10.1016/j.msec.2019.109938es
dc.identifier.urihttp://hdl.handle.net/11447/6361
dc.language.isoenes
dc.subjectPolyelectrolyteses
dc.subjectAmino acid functionalizationes
dc.subjectEnzyme adsorptiones
dc.subjectGlucose 6-phosphate dehydrogenasees
dc.subjectSurfaceses
dc.titleSurfaces based on amino acid functionalized polyelectrolyte films towards active surfaces for enzyme immobilizationes
dc.typeArticlees
dcterms.sourceMaterials Science and Engineering: Ces

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