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Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells

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dc.contributor.author Palacios-Moreno, Juan
dc.contributor.author Rubio, Cecilia
dc.contributor.author Quilhot, Wanda
dc.contributor.author Cavieres, M. Fernanda
dc.contributor.author Peña, Eduardo de la
dc.contributor.author Quiñones, Natalia V.
dc.contributor.author Díaz, Hugo
dc.contributor.author Carrión, Flavio
dc.contributor.author Henríquez-Roldán, Carlos F.
dc.contributor.author Weinstein-Oppenheimer, Caroline R.
dc.date.accessioned 2020-03-09T17:19:46Z
dc.date.available 2020-03-09T17:19:46Z
dc.date.issued 2019
dc.identifier.citation Biol Res 52, 55 (2019).
dc.identifier.uri http://hdl.handle.net/11447/3130
dc.identifier.uri http://dx.doi.org/10.1186/s40659-019-0261-4
dc.description.abstract Background: Epanorin (EP) is a secondary metabolite of the Acarospora lichenic species. EP has been found in lichenic extracts with antimicrobial activity, and UV-absorption properties have been described for closely related molecules; however, its antiproliferative activity in cancer cells has not yet been explored. It has been hypothesized that EP inhibits cancer cell growth. MCF-7 breast cancer cells, normal fibroblasts, and the non-transformed HEK-293 cell line were exposed to increasing concentrations of EP, and proliferation was assessed by the sulforhodamine-B assay. Results: MCF-7 cells exposed to EP were examined for cell cycle progression using flow cytometry, and DNA fragmentation was examined using the TUNEL assay. In addition, EP's mutagenic activity was assessed using the Salmonella typhimurium reverse mutation assay. The data showed that EP inhibits proliferation of MCF-7 cells, and it induces cell cycle arrest in G0/G1 through a DNA fragmentation-independent mechanism. Furthermore, EP's lack of overt cytotoxicity in the normal cell line HEK-293 and human fibroblasts in cell culture is supported by the absence of mutagenic activity of EP. Conclusion: EP emerges as a suitable molecule for further studies as a potential antineoplastic agent.
dc.format.extent 11 p.
dc.language.iso en
dc.subject Epanorin
dc.subject Cancer
dc.subject Cytotoxicity
dc.subject Mutagenesis
dc.subject Cell cycle
dc.subject Apoptosis
dc.title Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells
dc.type Article


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