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Dynamics of the MRSA Population in a Chilean Hospital: a Phylogenomic Analysis (2000-2016)

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dc.contributor.authorMartínez, José
dc.contributor.authorPlanet, Paul
dc.contributor.authorSpencer, Maria
dc.contributor.authorRivas, Lina
dc.contributor.authorDíaz, Lorena
dc.contributor.authorMoustafa, Ahmed
dc.contributor.authorRiquelme, Roberto
dc.contributor.authorAlcalde, Manuel
dc.contributor.authorHanson, Blake
dc.contributor.authorCarvajal, Lina
dc.contributor.authorRincón, Sandra
dc.contributor.authorReyes, Jinnethe
dc.contributor.authorLam, Marusella
dc.contributor.authorCalderon, Juan
dc.contributor.authorAraos, Rafael
dc.contributor.authorGarcía, Patricia
dc.contributor.authorArias, César
dc.contributor.authorMunita, Jose M.
dc.date.accessioned2024-05-13T22:16:54Z
dc.date.available2024-05-13T22:16:54Z
dc.date.issued2023
dc.description.abstractThe global dissemination of methicillin-resistant Staphylococcus aureus (MRSA) is associated with the emergence and establishment of clones in specific geographic areas. The Chilean-Cordobes clone (ChC) (ST5-SCCmecI) has been the predominant MRSA clone in Chile since its first description in 1998, despite the report of other emerging MRSA clones in recent years. Here, we characterize the evolutionary history of MRSA from 2000 to 2016 in a Chilean tertiary health care center using phylogenomic analyses. We sequenced 469 MRSA isolates collected between 2000 and 2016. We evaluated the temporal trends of the circulating clones and performed a phylogenomic reconstruction to characterize the clonal dynamics. We found a significant increase in the diversity and richness of sequence types (STs; Spearman r = 0.8748, P < 0.0001) with a Shannon diversity index increasing from 0.221 in the year 2000 to 1.33 in 2016, and an effective diversity (Hill number; q = 2) increasing from 1.12 to 2.71. The temporal trend analysis revealed that in the period 2000 to 2003 most of the isolates (94.2%; n = 98) belonged to the ChC clone. However, since then, the frequency of the ChC clone has decreased over time, accounting for 52% of the collection in the 2013 to 2016 period. This decline was accompanied by the rise of two emerging MRSA lineages, ST105-SCCmecII and ST72-SCCmecVI. In conclusion, the ChC clone remains the most frequent MRSA lineage, but this lineage is gradually being replaced by several emerging clones, the most important of which is clone ST105-SCCmecII. To the best of our knowledge, this is the largest study of MRSA clonal dynamics performed in South America. IMPORTANCE Methicillin-resistant Staphylococcus aureus (MRSA) is a major public health pathogen that disseminates through the emergence of successful dominant clones in specific geographic regions. Knowledge of the dissemination and molecular epidemiology of MRSA in Latin America is scarce and is largely based on small studies or more limited typing techniques that lack the resolution to represent an accurate description of the genomic landscape. We used whole-genome sequencing to study 469 MRSA isolates collected between 2000 and 2016 in Chile providing the largest and most detailed study of clonal dynamics of MRSA in South America to date. We found a significant increase in the diversity of MRSA clones circulating over the 17-year study period. Additionally, we describe the emergence of two novel clones (ST105-SCCmecII and ST72-SCCmecVI), which have been gradually increasing in frequency over time. Our results drastically improve our understanding of the dissemination and update our knowledge about MRSA in Latin America.
dc.description.versionPublicada
dc.identifier.citationMartínez JRW, Planet PJ, Spencer-Sandino M, Rivas L, Díaz L, Moustafa AM, Quesille-Villalobos A, Riquelme-Neira R, Alcalde-Rico M, Hanson B, Carvajal LP, Rincón S, Reyes J, Lam M, Calderon JF, Araos R, García P, Arias CA, Munita JM. Dynamics of the MRSA Population in a Chilean Hospital: a Phylogenomic Analysis (2000-2016). Microbiol Spectr. 2023 Aug 17;11(4):e0535122. doi: 10.1128/spectrum.05351-22
dc.identifier.doihttps://doi.org/10.1128/spectrum.05351-22
dc.identifier.urihttps://hdl.handle.net/11447/8743
dc.language.isoen
dc.subjectStaphylococcus aureus
dc.subjectClonality
dc.subjectGenomics
dc.subjectMethicillin resistance
dc.subjectPhylogenomic analysis
dc.titleDynamics of the MRSA Population in a Chilean Hospital: a Phylogenomic Analysis (2000-2016)
dc.typeArticle
dcterms.accessRightsAcceso Abierto
dcterms.sourceMicrobiology spectrum
dspace.entity.typePublication
relation.isAuthorOfPublicationec52b0bf-d0bc-4844-9531-eca4a65f2b8e
relation.isAuthorOfPublication.latestForDiscoveryec52b0bf-d0bc-4844-9531-eca4a65f2b8e

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