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Development of a New Genus-Specific Quantitative Real-Time PCR Assay for the Diagnosis of Scrub Typhus in South America

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dc.contributor.authorJiang, Ju
dc.contributor.authorMartínez, Constanza
dc.contributor.authorWeitzel, Thomas
dc.contributor.authorFarris, Christina
dc.contributor.authorAcosta, Gerardo
dc.contributor.authorAbarca, Katia
dc.contributor.authorRichards, Allen
dc.date.accessioned2023-04-05T13:46:02Z
dc.date.available2023-04-05T13:46:02Z
dc.date.issued2022
dc.description.abstractScrub typhus is a potentially severe rickettsiosis, caused by Orientia tsutsugamushi in the Asia-Pacific region. Recently, however, two distinct pathogens, “Candidatus Orientia chuto” and “Candidatus Orientia chiloensis”, have been discovered in the Middle East and South America, respectively. Since the novel pathogens differ significantly from O. tsutsugamushi, many established diagnostic methods are unreliable. This work describes the development and validation of a new quantitative real-time PCR (qPCR) assay (Orien16S) for the detection of all known Orientia species. Based on a 94 bp sequence of the 16S rRNA gene (rrs), Orien16S recognized DNA samples from O. tsutsugamushi (n = 41), Ca. O. chiloensis (n = 5), and Ca. O. chuto (n = 1), but was negative for DNA preparations from closely related rickettsiae and other members of the order Rickettsiales (n = 22) as well as unrelated bacterial species (n = 11). After its implementation in Chile, the assay was verified, correctly identifying all tested eschar and buffy coat samples (n = 28) of clinical suspected cases. Furthermore, Orien16S detected Orientia DNA in trombiculid mites collected in endemic regions in southern Chile. The presented novel qPCR assay provides a useful tool for detecting Orientia and diagnosing scrub typhus from all geographical regions.
dc.description.versionVersión publicada
dc.format.extentFONDECYT
dc.identifier.citationJiang J, Martínez-Valdebenito C, Weitzel T, Farris CM, Acosta-Jamett G, Abarca K, Richards AL. Development of a New Genus-Specific Quantitative Real-Time PCR Assay for the Diagnosis of Scrub Typhus in South America. Front Med (Lausanne). 2022 Apr 27;9:831045. doi: 10.3389/fmed.2022.831045
dc.identifier.doihttps://doi.org/10.3389/fmed.2022.831045
dc.identifier.urihttps://repositorio.udd.cl/handle/11447/7261
dc.language.isoen
dc.subjectCandidatus Orientia chiloensis
dc.subjectChile
dc.subjectOrien16S
dc.subjectOrientia
dc.subjectSouth America
dc.subjectMolecular diagnostic techniques
dc.subjectQuantitative real-time PCR (qPCR)
dc.subjectScrub typhus
dc.titleDevelopment of a New Genus-Specific Quantitative Real-Time PCR Assay for the Diagnosis of Scrub Typhus in South America
dc.typeArticle
dcterms.accessRightsAcceso abierto
dcterms.sourceFrontiers in medicine
dspace.entity.typePublication

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