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Deep immunophenotyping reveals biomarkers of multisystemic inflammatory syndrome in children in a Latin American cohort

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dc.contributor.authorRey, Emma
dc.contributor.authorEspinosa, Yazmin
dc.contributor.authorAstudillo, Camila
dc.contributor.authorJimena, Lina
dc.contributor.authorHormazábal, Juan
dc.contributor.authorNoguera, Loreani
dc.contributor.authorCofré, Fernanda
dc.contributor.authorPiñera, Cecilia
dc.contributor.authorGonzález, Ricardo
dc.contributor.authorBataszew, Alexander
dc.contributor.authorMuñoz, Paula
dc.contributor.authorBenadof, Dona
dc.contributor.authorÁlvarez, Patricia
dc.contributor.authorAcevedo, Valeria
dc.contributor.authorVial, Pablo
dc.contributor.authorVial, Cecilia
dc.contributor.authorPoli, Cecilia
dc.date.accessioned2023-04-26T19:28:44Z
dc.date.available2023-04-26T19:28:44Z
dc.date.issued2022
dc.description.abstractBackground: Multisystemic inflammatory syndrome in children (MIS-C) is a life-threatening disease that occurs 2-5 weeks after severe acute respiratory syndrome coronavirus 2 exposure and is characterized by severe multisystemic inflammation. Early recognition of MIS-C is key to prognosis; therefore, establishing clinical and laboratory biomarkers that predict complications is urgently needed. Objective: We characterized the immune response and clinical features of patients with acute MIS-C and determined biomarkers of disease in a cohort of 42 Latin American patients. Methods: Immune characterization was performed using flow cytometry from peripheral mononuclear cells and severe acute respiratory syndrome coronavirus 2-specific humoral and cellular response was performed using flow cytometry, enzyme-linked immunospot, enzyme-linked immunosorbent assay, and neutralizing antibody assays. Results: MIS-C is characterized by robust T-cell activation and cytokine storm. We uncovered that while C-X-C motif chemokine ligand (CXCL) 9, IL-10, CXCL8, CXCL10, IL-6, and IL-18 are significantly elevated in patients with shock, while CCL5 was increased in milder disease. Monocyte dysregulation was specifically associated with KD-like MIS-C. Interestingly, MIS-C patients show a natural killer cell degranulation defect that is persistent after 6 months of disease presentation, suggesting it could underlie disease susceptibility. Most MIS-C had gastrointestinal involvement, and higher levels of neopterin were identified in their stools, potentially representing a biomarker of intestinal inflammation in MIS-C. Severe acute respiratory syndrome coronavirus 2-specific cellular response and neutralizing antibodies were identifiable in convalescent MIS-C patients, suggesting sustained immunity. Conclusion: Clinical characterization and comprehensive immunophenotyping of Chilean MIS-C cohort provide valuable insights in understanding immune dysregulation in MIS-C and identify relevant biomarkers of disease that could be used to predict severity and organ involvement.
dc.description.versionVersión Publicada
dc.identifier.citationRey-Jurado E, Espinosa Y, Astudillo C, Jimena Cortés L, Hormazabal J, Noguera LP, Cofré F, Piñera C, González R, Bataszew A, Muñoz Venturelli P, Benadof D, Álvarez P, Acevedo V, Vial P, Vial C, Poli MC. Deep immunophenotyping reveals biomarkers of multisystemic inflammatory syndrome in children in a Latin American cohort. J Allergy Clin Immunol. 2022 Nov;150(5):1074-1085.e11. doi: 10.1016/j.jaci.2022.09.006
dc.identifier.doihttps://doi.org/10.1016/j.jaci.2022.09.006
dc.identifier.urihttps://repositorio.udd.cl/handle/11447/7403
dc.language.isoen
dc.subjectCOVID-19
dc.subjectNK cell deficiency
dc.subjectBiomarkers
dc.subjectInflammation
dc.subjectMultisystemic inflammatory syndrome in children
dc.titleDeep immunophenotyping reveals biomarkers of multisystemic inflammatory syndrome in children in a Latin American cohort
dc.typeArticle
dcterms.accessRightsAcceso abierto
dcterms.accessRightsAcceso Abierto
dcterms.sourceThe Journal of allergy and clinical immunology
dspace.entity.typePublication

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