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Viral shedding and viraemia of Andes virus during acute hantavirus infection: a prospective study

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dc.contributor.authorFerrés, Marcela
dc.contributor.authorMartínez, Constanza
dc.contributor.authorHenriquez, Carolina
dc.contributor.authorMarco, Claudia
dc.contributor.authorBarrera, Aldo
dc.contributor.authorPalma, Carlos
dc.contributor.authorBarriga, Gonzalo
dc.contributor.authorCuiza, Analia
dc.contributor.authorFerreira, Leonila
dc.contributor.authorRioseco, María
dc.contributor.authorCalvo, Mario
dc.contributor.authorFritz, Ricardo
dc.contributor.authorBravo, Sebastián
dc.contributor.authorBruhn, Alejandro
dc.contributor.authorGraf, Jerónimo
dc.contributor.authorLlancaqueo, Alvaro
dc.contributor.authorRivera, Gonzalo
dc.contributor.authorCerda, Carolina
dc.contributor.authorTischler, Nicole
dc.contributor.authorValdivieso, Francisca
dc.contributor.authorVial, Pablo
dc.contributor.authorMertz, Gregory
dc.contributor.authorVial Cox, María Cecilia
dc.contributor.authorLe Corre, Nicole
dc.date.accessioned2025-01-28T15:59:25Z
dc.date.available2025-01-28T15:59:25Z
dc.date.issued2024
dc.description.abstractBackground: Andes virus (ANDV) is a zoonotic Orthohantavirus leading to hantavirus cardiopulmonary syndrome. Although most transmissions occur through environmental exposure to rodent faeces and urine, rare person-to-person transmission has been documented, mainly for close contacts. This study investigates the presence and infectivity of ANDV in body fluids from confirmed cases and the duration of viraemia. Methods: In this prospective study, 131 participants with confirmed ANDV infection were enrolled in Chile in a prospective study between 2008 and 2022. Clinical samples (buffy coat, plasma, gingival crevicular fluid [GCF], saliva, nasopharyngeal swabs [NPS], and urine) were collected weekly for 3 weeks together with clinical and epidemiological data. Samples were categorised as acute or convalescent (up to and after 16 days following onset of symptoms). Infectivity of positive fluids was assessed after the culture of samples on Vero E6 cells and use of flow cytometry assays to determine the production of ANDV nucleoprotein. Findings: ANDV RNA was detected in 100% of buffy coats during acute phase, declining to 95% by day 17, and to 93% between days 23-29. ANDV RNA in GCF and saliva decreased from 30% and 12%, respectively, during the acute phase, to 12% and 11% during the convalescent phase. Successful infectivity assays of RT-qPCR-positive fluids, including GCF, saliva, NPS, and urine, were observed in 18 (42%) of 43 samples obtained during the acute phase of infection. After re-culture, the capacity to infect Vero E6 cells was maintained in 16 (89%) of 18 samples. Severity was associated with the presence of ANDV RNA in one or more fluids besides blood (odds ratio 2·58 [95% CI 1·42-5·18]). Interpretation: ANDV infection is a systemic and viraemic infection, that affects various organs. The presence of infectious particles in body fluids contributes to our understanding of potential mechanisms for person-to-person transmission, supporting the development of preventive strategies. Detection of ANDV RNA in additional fluids at hospital admission is a predictor of disease severity. Funding: National Institutes of Health and Agencia de Investigación y Desarrollo.
dc.description.versionVersión Aceptada
dc.identifier.citationFerrés M, Martínez-Valdebenito C, Henriquez C, Marco C, Angulo J, Barrera A, Palma C, Barriga Pinto G, Cuiza A, Ferreira L, Rioseco ML, Calvo M, Fritz R, Bravo S, Bruhn A, Graf J, Llancaqueo A, Rivera G, Cerda C, Tischler N, Valdivieso F, Vial P, Mertz G, Vial C, Le Corre N. Viral shedding and viraemia of Andes virus during acute hantavirus infection: a prospective study. Lancet Infect Dis. 2024 Jul;24(7):775-782. doi: 10.1016/S1473-3099(24)00142-7. Epub 2024 Apr 3. Erratum in: Lancet Infect Dis. 2024 Jul;24(7):e419. doi: 10.1016/S1473-3099(24)00299-8
dc.identifier.doihttps://doi.org/10.1016/S1473-3099(24)00142-7
dc.identifier.urihttps://hdl.handle.net/11447/9720
dc.language.isoen
dc.subjectHantavirus Infections / epidemiology
dc.subjectHantavirus Infections / transmission
dc.subjectHantavirus Infections / virology
dc.subjectChile / epidemiology
dc.titleViral shedding and viraemia of Andes virus during acute hantavirus infection: a prospective study
dc.typeArticle
dcterms.accessRightsAcceso Abierto
dcterms.sourceThe Lancet. Infectious diseases
dspace.entity.typePublication
relation.isAuthorOfPublicationc60c673d-2a3e-4c8f-af8c-cb6efb9c4b0e
relation.isAuthorOfPublication4cc9451a-447d-42c8-8e68-8669895ed0a0
relation.isAuthorOfPublication.latestForDiscoveryc60c673d-2a3e-4c8f-af8c-cb6efb9c4b0e

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