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Protocol for the study of Connexin and DNA interactions

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dc.contributor.authorFernández-Olivares, Ainoa
dc.contributor.authorRetamal, Mauricio A.
dc.date.accessioned2024-12-31T14:43:14Z
dc.date.available2024-12-31T14:43:14Z
dc.date.issued2024
dc.description.abstractConnexins (Cxs) are transmembrane proteins which form hemichannels and gap junction channels at the plasma membrane. These channels allow the exchange of ions and molecules between the intra- and extracellular space and between cytoplasm of adjacent cells, respectively. The channel function of Cx assemblies has been extensively studied; however, “noncanonical” functions have emerged in the last few decades and have capture the attentions of many researchers, including the role of some Cxs as gene modulators or transcription factors. In this chapter, we describe a protocol to study the interaction of Cx46 with DNA in HeLa cells. These methods can facilitate understanding the role of Cxs in physiological processes and pathological mechanisms, including, for example, the contribution of Cx46 in maintaining stemness of glioma cancer stem cells
dc.description.versionVersión enviada
dc.format.extent13 p.
dc.identifier.citationFernandez-Olivares, A., Retamal, M.A. (2024). Protocol for the Study of Connexin and DNA Interactions. In: Mammano, F., Retamal, M. (eds) Connexin Hemichannels. Methods in Molecular Biology, vol 2801. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3842-2_10
dc.identifier.doihttps://doi.org/10.1007/978-1-0716-3842-2_10
dc.identifier.urihttps://hdl.handle.net/11447/9546
dc.language.isoen
dc.subjectConnexins
dc.subjectChromatin Immunoprecipitation (Chip)
dc.subjectDNA interactions
dc.subjectOmmunoprecipitation
dc.subjectCx46
dc.titleProtocol for the study of Connexin and DNA interactions
dc.typeBook chapter
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relation.isAuthorOfPublication.latestForDiscovery01e4a6fa-e55f-47c5-a1b6-383d5fc6f474

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