Two-Month Voluntary Ethanol Consumption Promotes Mild Neuroinflammation in the Cerebellum but Not in the Prefrontal Cortex, Hippocampus, or Striatum of Mice

Berríos, Pablo; Núñez, Sarah; Castañeda, Justine; Gallardo, Javiera; Bono, María; Ezquer, Fernando

Publication:
Two-Month Voluntary Ethanol Consumption Promotes Mild Neuroinflammation in the Cerebellum but Not in the Prefrontal Cortex, Hippocampus, or Striatum of Mice

dc.contributor.author	Berríos, Pablo
dc.contributor.author	Núñez, Sarah
dc.contributor.author	Castañeda, Justine
dc.contributor.author	Gallardo, Javiera
dc.contributor.author	Bono, María
dc.contributor.author	Ezquer, Fernando
dc.date.accessioned	2025-01-09T16:54:00Z
dc.date.available	2025-01-09T16:54:00Z
dc.date.issued	2024
dc.description.abstract	Chronic ethanol exposure often triggers neuroinflammation in the brain's reward system, potentially promoting the drive for ethanol consumption. A main marker of neuroinflammation is the microglia-derived monocyte chemoattractant protein 1 (MCP1) in animal models of alcohol use disorder in which ethanol is forcefully given. However, there are conflicting findings on whether MCP1 is elevated when ethanol is taken voluntarily, which challenges its key role in promoting motivation for ethanol consumption. Here, we studied MCP1 mRNA levels in areas implicated in consumption motivation-specifically, the prefrontal cortex, hippocampus, and striatum-as well as in the cerebellum, a brain area highly sensitive to ethanol, of C57BL/6 mice subjected to intermittent and voluntary ethanol consumption for two months. We found a significant increase in MCP1 mRNA levels in the cerebellum of mice that consumed ethanol compared to controls, whereas no significant changes were observed in the prefrontal cortex, hippocampus, or striatum or in microglia isolated from the hippocampus and striatum. To further characterize cerebellar neuroinflammation, we measured the expression changes in other proinflammatory markers and chemokines, revealing a significant increase in the proinflammatory microRNA miR-155. Notably, other classical proinflammatory markers, such as TNFα, IL6, and IL-1β, remained unaltered, suggesting mild neuroinflammation. These results suggest that the onset of neuroinflammation in motivation-related areas is not required for high voluntary consumption in C57BL/6 mice. In addition, cerebellar susceptibility to neuroinflammation may be a trigger to the cerebellar degeneration that occurs after chronic ethanol consumption in humans.
dc.description.version	Versión Publicada
dc.identifier.citation	Berríos-Cárcamo P, Núñez S, Castañeda J, Gallardo J, Bono MR, Ezquer F. Two-Month Voluntary Ethanol Consumption Promotes Mild Neuroinflammation in the Cerebellum but Not in the Prefrontal Cortex, Hippocampus, or Striatum of Mice. Int J Mol Sci. 2024 Apr 10;25(8):4173. doi: 10.3390/ijms25084173
dc.identifier.doi	https://doi.org/10.3390/ijms25084173
dc.identifier.uri	https://hdl.handle.net/11447/9608
dc.language.iso	en
dc.title	Two-Month Voluntary Ethanol Consumption Promotes Mild Neuroinflammation in the Cerebellum but Not in the Prefrontal Cortex, Hippocampus, or Striatum of Mice
dc.type	Article
dcterms.accessRights	Acceso Abierto
dcterms.source	International journal of molecular sciences
dspace.entity.type	Publication
relation.isAuthorOfPublication	c74766c7-dad8-4d11-b35d-01da0664f3da
relation.isAuthorOfPublication.latestForDiscovery	c74766c7-dad8-4d11-b35d-01da0664f3da