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Exploring HLA-C methylation patterns and nutritional status in Kichwa mothers and infants from Tena, Ecuador

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dc.contributor.authorVelastegui, Erick
dc.contributor.authorFalconí, Isaac
dc.contributor.authorGarcia, Valeria
dc.contributor.authorMunizaga, Gabriela
dc.contributor.authorMatias, Carmen
dc.contributor.authorSegura, Yaritza
dc.contributor.authorAlcivar, Kerly
dc.contributor.authorValencia, Luz
dc.contributor.authorVera, Edwin
dc.contributor.authorMuñoz, Mindy
dc.contributor.authorVanden Berghe, Wim
dc.contributor.authorLebeer, Sarah
dc.contributor.authorOrellana, Andrea
dc.date.accessioned2025-01-16T19:00:52Z
dc.date.available2025-01-16T19:00:52Z
dc.date.issued2024
dc.description.abstractEnvironment and lifestyle can affect the epigenome passed down from generation to generation. A mother's nutrition can impact the methylation levels of her offspring's epigenome, but it's unclear which genes may be affected by malnutrition during gestation or early development. In this study, we examined the levels of methylated GC in the promoter region of HLA-C in mothers and infants from the Kichwa community in Ecuador. To do this, we analyzed saliva samples using bisulfite DNA sequencing. While we did not observe any significant differences in the mean methylation percentages in exon 1 of HLA-C between mothers and their infants after the first two years of lactation and life, respectively, we did find that infants tended to increase their methylation level during the first two years of life, while mothers tended to decrease it after the first two years of breastfeeding. When we compared methylation levels between mothers and infants using an ANOVA/posthoc Tukey test, we found that the average methylation for the entire population was less than 3% at T1 and T2. Although there was a tendency for infants to have higher methylation levels during their first two years of life and for mothers to have lower methylation levels after the first two years of breastfeeding, the mean values were not significantly different. However, we found a significant difference when we contrasted the data using a Kruskal-Wallis test at 0.05 for T1 AND T2 (p-value: 0.0148). Specifically, mothers had an average of X̅ = 2.06% and sons had X̅ = 1.57% at T2 (p-value: 0.7227), while the average for mothers was X̅ = 1.83% and for sons X̅ =1.77%. Finally, we identified three CpG motif nucleotide positions (32-33, 43-44, and 96-97) along the 122 bp analysis of HLA-C exon one, which was found to retain methylation patterns over time and is inherited from mother to offspring. Finally, our small pilot study did not reveal significant correlations between maternal and offspring nutritional status and DNA methylation levels of HLA-C exon one.
dc.description.versionVersión Publicada
dc.identifier.citationVelastegui E, Falconí IB, Garcia VI, Munizaga G, Matias de la Cruz C, Segura Y, Alcivar K, Valencia L, Vera E, Muñoz MS, Vanden Berghe W, Lebeer S, Orellana-Manzano A. Exploring HLA-C methylation patterns and nutritional status in Kichwa mothers and infants from Tena, Ecuador. Front Med (Lausanne). 2024 Aug 27;11:1356646. doi: 10.3389/fmed.2024.1356646
dc.identifier.doihttps://doi.org/10.3389/fmed.2024.1356646
dc.identifier.urihttps://hdl.handle.net/11447/9664
dc.language.isoen
dc.subjectHLA-C
dc.subjectEpigenetic
dc.subjectInheritance
dc.subjectMethylation
dc.subjectNutrition
dc.titleExploring HLA-C methylation patterns and nutritional status in Kichwa mothers and infants from Tena, Ecuador
dc.typeArticle
dcterms.accessRightsAcceso Abierto
dcterms.sourceFrontiers in Medicine
dspace.entity.typePublication

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