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Identification of Circulating lncRNAs Associated with Gallbladder Cancer Risk by Tissue-Based Preselection, Cis-eQTL Validation, and Analysis of Association with Genotype-Based Expression

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dc.contributor.authorBlandino, Alice
dc.contributor.authorScherer, Dominique
dc.contributor.authorRounge, Trine B.
dc.contributor.authorUmu, Sinan U.
dc.contributor.authorBoekstegers, Felix
dc.contributor.authorBarahona Ponce, Carol
dc.contributor.authorMarcelain, Katherine
dc.contributor.authorGárate-Calderón, Valentina
dc.contributor.authorWaldenberger, Melanie
dc.contributor.authorMorales, Erik
dc.contributor.authorRojas, Armando
dc.contributor.authorMuñoz, César
dc.contributor.authorRetamales, Javier
dc.contributor.authorToro, Gonzalo de
dc.contributor.authorBarajas, Olga
dc.contributor.authorRivera, María Teresa
dc.contributor.authorCortés, Analía
dc.contributor.authorLoader, Denisse
dc.contributor.authorSaavedra, Javiera
dc.contributor.authorGutiérrez, Lorena
dc.contributor.authorOrtega, Alejandro
dc.contributor.authorBertrán, María Enriqueta
dc.contributor.authorGabler, Fernando
dc.contributor.authorCampos, Mónica
dc.contributor.authorAlvarado, Juan
dc.contributor.authorFabrizio Moisán 18, Loreto Spencer 18, Bruno Nervi 19, Daniel E Carvajal-Hausdorf
dc.contributor.authorSpencer, Loreto
dc.contributor.authorNervi, Bruno
dc.contributor.authorCarvajal-Hausdorf, Daniel
dc.contributor.authorLosada, Héctor
dc.contributor.authorAlmau, Mauricio
dc.contributor.authorFernández, Plinio
dc.contributor.authorGallegos, Iván
dc.contributor.authorOlloquequi, Jordi
dc.contributor.authorFuentes-Guajardo, Macarena
dc.contributor.authorGonzález -Jose, Rolando
dc.contributor.authorBortolini, María Cátira
dc.contributor.authorGallo, Carla
dc.contributor.authorRuíz Linares, Andrés
dc.contributor.authorRothhammer, Francisco
dc.contributor.authorBermejo, Justo Lorenzo
dc.date.accessioned2023-12-04T20:45:58Z
dc.date.available2023-12-04T20:45:58Z
dc.date.issued2022
dc.description.abstractLong noncoding RNAs (lncRNAs) play key roles in cell processes and are good candidates for cancer risk prediction. Few studies have investigated the association between individual genotypes and lncRNA expression. Here we integrate three separate datasets with information on lncRNA expression only, both lncRNA expression and genotype, and genotype information only to identify circulating lncRNAs associated with the risk of gallbladder cancer (GBC) using robust linear and logistic regression techniques. In the first dataset, we preselect lncRNAs based on expression changes along the sequence "gallstones → dysplasia → GBC". In the second dataset, we validate associations between genetic variants and serum expression levels of the preselected lncRNAs (cis-lncRNA-eQTLs) and build lncRNA expression prediction models. In the third dataset, we predict serum lncRNA expression based on individual genotypes and assess the association between genotype-based expression and GBC risk. AC084082.3 and LINC00662 showed increasing expression levels (p-value = 0.009), while C22orf34 expression decreased in the sequence from gallstones to GBC (p-value = 0.04). We identified and validated two cis-LINC00662-eQTLs (r2 = 0.26) and three cis-C22orf34-eQTLs (r2 = 0.24). Only LINC00662 showed a genotyped-based serum expression associated with GBC risk (OR = 1.25 per log2 expression unit, 95% CI 1.04-1.52, p-value = 0.02). Our results suggest that preselection of lncRNAs based on tissue samples and exploitation of cis-lncRNA-eQTLs may facilitate the identification of circulating noncoding RNAs linked to cancer risk.
dc.description.versionVersión publicada
dc.format.extent15 p.
dc.identifier.citationBlandino, A.; Scherer, D.; Rounge, T.B.; Umu, S.U.; Boekstegers, F.; Barahona Ponce, C.; Marcelain, K.; Gárate-Calderón, V.; Waldenberger, M.; Morales, E.; et al. Identification of Circulating lncRNAs Associated with Gallbladder Cancer Risk by Tissue-Based Preselection, Cis-eQTL Validation, and Analysis of Association with Genotype-Based Expression. Cancers 2022, 14, 634. https://doi.org/10.3390/cancers14030634
dc.identifier.doihttps://doi.org/10.3390/cancers14030634
dc.identifier.urihttps://repositorio.udd.cl/handle/11447/8114
dc.language.isoen
dc.subjecteQTLs
dc.subjectGallbladder cancer
dc.subjectGenetic association study
dc.subjectIncRNAs
dc.subjectMolecular phenotypes
dc.titleIdentification of Circulating lncRNAs Associated with Gallbladder Cancer Risk by Tissue-Based Preselection, Cis-eQTL Validation, and Analysis of Association with Genotype-Based Expression
dc.typeArticle
dcterms.accessRightsAcceso abierto
dcterms.sourceCancers
dspace.entity.typePublication
relation.isAuthorOfPublication8e2ee03b-003a-4e24-b577-290a5cd449c1
relation.isAuthorOfPublication.latestForDiscovery8e2ee03b-003a-4e24-b577-290a5cd449c1

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