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Gut Microbiota-Derived Extracellular Vesicles Influence Alcohol Intake Preferences in Rats

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dc.contributor.authorDíaz-Ubilla, Macarena
dc.contributor.authorFigueroa-Valdés, Aliosha I.
dc.contributor.authorTobar, Hugo E.
dc.contributor.authorQuintanilla, María Elena
dc.contributor.authorDíaz, Eugenio
dc.contributor.authorMorales, Paola
dc.contributor.authorBerríos-Cárcamo, Pablo
dc.contributor.authorSantapau, Daniela
dc.contributor.authorGallardo, Javiera
dc.contributor.authorGregorio, Cristian de
dc.contributor.authorUgalde, Juan
dc.contributor.authorRojas, Carolina
dc.contributor.authorGonzalez-Madrid, Antonia
dc.contributor.authorEzquer, Marcelo
dc.contributor.authorIsrael, Yedy
dc.contributor.authorAlcayaga-Miranda, Francisca
dc.contributor.authorEzquer, Fernando
dc.date.accessioned2026-08-27T21:47:10Z
dc.date.available2026-08-27T21:47:10Z
dc.date.issued2025
dc.description.abstractGrowing preclinical and clinical evidence suggests a link between gutmicrobiota dysbiosis and problematic alcohol consumption. Extracellular vesicles (EVs) are key mediators involved in bacteria-to-host communication. However, their potential role in mediating addictive behaviour remains unexplored. This study investigates the role of gut microbiota-derived bacterial extracellular vesicles (bEVs) in driving high alcohol consumption. bEVs were isolated from the gut microbiota of a high alcoholdrinking rat strain (UChB rats), either ethanol-naïve or following chronic alcohol consumption and administered intraperitoneally or orally to alcohol-rejecting male and femaleWistar rats. Both types of UChB-derived bEVs increased Wistar’s voluntary alcohol consumption (three bottle choice test) up to 10-fold (p < 0.0001), indicating that bEVs are able and sufficient to transmit drinking behaviour across different rat strains. Molecular analysis revealed that bEVs administration did not induce systemic or brain inflammation in the recipient animals, suggesting that the increased alcohol intake triggered by UChB-derived bEVs operates through an inflammation-independent mechanism. Furthermore,we demonstrate that the vagus nerve mediates the bEV-induced increase in alcohol consumption, as bilateral vagotomy completely abolished the high drinking behaviour induced by both intraperitoneally injected and orally administered bEVs. Thus, this study identifies bEVs as a novel mechanism underlying gut microbiota-induced high alcohol intake in a vagus nerve-dependent manner.
dc.description.versionVersión publicada
dc.format.extent19 p.
dc.identifier.citationDíaz-Ubilla, M., Figueroa-Valdés, A.I., Tobar, H.E., Quintanilla, M.E., Díaz, E., Morales, P., Berríos-Cárcamo, P., Santapau, D., Gallardo, J., de Gregorio, C., Ugalde, J., Rojas, C., Gonzalez-Madrid, A., Ezquer, M., Israel, Y., Alcayaga-Miranda, F. and Ezquer, F. (2025), Gut Microbiota-Derived Extracellular Vesicles Influence Alcohol Intake Preferences in Rats. J Extracell Vesicles., 14: e70059. https://doi.org/10.1002/jev2.70059
dc.identifier.doihttps://doi.org/10.1002/jev2.70059
dc.identifier.urihttps://hdl.handle.net/11447/11045
dc.language.isoen
dc.subjectAddictive behaviour
dc.subjectAlcohol consumption
dc.subjectBacterial vesicles
dc.subjectbEVs
dc.subjectGut microbiota
dc.subjectInflammation
dc.subjectMicrobiota-derived EVs
dc.subjectVagus nerve
dc.titleGut Microbiota-Derived Extracellular Vesicles Influence Alcohol Intake Preferences in Rats
dc.typeArticle
dcterms.accessRightsAcceso abierto
dcterms.sourceJournal of Extracellular Vesicles
dspace.entity.typePublication

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